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bioRxiv · 10.1101/125930

Toward D-Peptide Biosynthesis: Elongation Factor P Enables Ribosomal Incorporation Of Consecutive D-Amino Acids

Huang, P.-Y.·Wang, F.·Narasimhan, K.·Chatman, K.·Aach, J.·Trauger, S. A.·Spoering, R.·Church, G. M.
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Abstract

To maintain stereospecific biochemistry in cells, living organisms have evolved mechanisms to exclude D-amino acids (DAA) in their protein synthesis machinery, which also limits our exploration of the realm of mirror-image molecules. Here, we show that high affinity between EF-Tu and aminoacyl-tRNA promotes D-amino acid incorporation. More strikingly, Elongation Factor P efficiently resolves peptidyl transferase stalling between two consecutive D-amino acids, and hence enables the translation of D-peptides.

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Huang, P.-Y., Wang, F., Narasimhan, K., Chatman, K., Aach, J., Trauger, S. A., Spoering, R., Church, G. M.. 2017-04-10. Toward D-Peptide Biosynthesis: Elongation Factor P Enables Ribosomal Incorporation Of Consecutive D-Amino Acids. https://doi.org/10.1101/125930

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