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bioRxiv · 10.1101/117663

Genome-wide Segregation of Single Nucleotide and Structural Variants into Single Leukemia Cells

Easton, J.·Gonzalez-Pena, V.·Yergeau, D.·Ma, X.·Gawad, C.
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Abstract

We present a new approach for determining comprehensive variant profiles of single cells using a microfluidic amplicon-based strategy. This method can be used to reconstruct the clonal architecture and mutational history of a malignancy using all classes and sizes of single nucleotide and structural variants, providing insights into the temporal changes in mutational classes and processes that led to the development of a cancer. Using this approach, we interrogated single cells from a patient with leukemia, determining that processes producing structural variation preceded single nucleotides changes in the development of that malignancy.

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Easton, J., Gonzalez-Pena, V., Yergeau, D., Ma, X., Gawad, C.. 2017-03-16. Genome-wide Segregation of Single Nucleotide and Structural Variants into Single Leukemia Cells. https://doi.org/10.1101/117663

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