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bioRxiv · 10.1101/110262

SAVE: A secure cloud-based pipeline for CRISPR pooled screen deconvolution

Jeong, H.-h.·Kim, S. Y.·Rosseaux, M. W.·Zoghbi, H. Y.·Liu, Z.
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Abstract

We present a user-friendly, cloud-based, data analysis pipeline for the deconvolution of pooled screening data. This tool, termed SAVE for Screening Analysis Visual Explorer, serves a dual purpose of extracting, clustering and analyzing raw next generation sequencing files derived from pooled screening experiments while at the same time presenting them in a user-friendly way on a secure web-based platform. Moreover, SAVE serves as a useful web-based analysis pipeline for reanalysis of pooled CRISPR screening datasets. Taken together, the framework described in this study is expected to accelerate development of web-based bioinformatics tool for handling all studies which include next generation sequencing data. SAVE is available at http://save.nrihub.org.

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Jeong, H.-h., Kim, S. Y., Rosseaux, M. W., Zoghbi, H. Y., Liu, Z.. 2017-02-21. SAVE: A secure cloud-based pipeline for CRISPR pooled screen deconvolution. https://doi.org/10.1101/110262

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