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bioRxiv · 10.1101/049668

Vasculogenesis Potential of Mesenchymal and Endothelial Stem Cells Isolated from Various Human Tissues.

Rokhsareh Rohban·Nathalie Etchart·Thomas Pieber
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Abstract

Neo vessel formation can be initiated by co-transplantation of mesenchymal stem cells (MSC) with endothelial colony-forming cells (ECFC). The two adult stem cell types can be isolated and expanded from a variety of tissues to be used for regenerative applications pro-angiogenesis.\n\nHere we performed a systematic study to evaluate the neo-vasculogenesis potential of MSC and ECFC isolated from various human tissues. MSC were isolated, purified and expanded in vitro from umbilical cord (UC) and umbilical cord blood (UCB), white adipose tissue (WAT), bone marrow (BM), and amniotic membrane of placenta (AMN).\n\nECFC were isolated from UC and UCB, WAT and peripheral blood (PB). ECFC and MSC and were co-transplanted admixed with extracellular matrix (Matrigel(R)) at a ratio of 5:1 to immune-deficient NSG mice, subcutaneously. The transplants were harvested after two weeks and the state of vessel formation and stability in the explants were investigated using immune-histochemical methods. The number of created micro-vessels was quantified using Hematoxylin & Eosin (H&E) staining followed by image J quantification.\n\nResults showed that ECFC and MSC possess variable capacity in contributing to neo-vasculogenesis. WAT and UCB-derived ECFC and WAT, UCB and BM-derived MSC are most potent cells in terms of neo-vessel formation in vivo. UC-derived ECFC and AMN-derived MSC have been shown to be least potent in contributing to neo-vasculogenesis. This variability might be due to variable phenotypes, or different genetic profiles of MSC and ECFC isolated from different tissues and/or donors.\n\nThe findings might give an insight into better regenerative strategies for neo-vessel formation in vivo.

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Rokhsareh Rohban, Nathalie Etchart, Thomas Pieber. 2016-04-21. Vasculogenesis Potential of Mesenchymal and Endothelial Stem Cells Isolated from Various Human Tissues.. https://doi.org/10.1101/049668

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