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bioRxiv · 10.1101/048959

Time-resolved dual RNA-Seq reveals extensive rewiring of lung epithelial and pneumococcal transcriptomes during early infection

Rieza Aprianto·Jelle Slager·Siger Holsappel·Jan-Willem Veening
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Abstract

Streptococcus pneumoniae (pneumococcus) is the main etiological agent of pneumonia. Pneumococcal pneumonia is initiated by bacterial adherence to lung epithelial cells. Infection to the epithelium is a disruptive interspecies interaction involving numerous transcription-mediated processes. Revealing transcriptional changes may provide valuable insights into pneumococcal disease. Dual RNA-Seq allows simultaneous monitoring of the transcriptomes of both host and pathogen. Here, we developed a time-resolved infection model of human lung alveolar epithelial cells by S. pneumoniae and assessed transcriptome changes by dual RNA-Seq. Our data provide new insights into host-microbe interactions and show that the epithelial glutathione-detoxification pathway is activated by bacterial presence. We observed that adherent pneumococci, not free-floating bacteria, access host-associated carbohydrates and repress innate immune responses. In conclusion, we provide a dynamic dual-transcriptomics overview of early pneumococcal infection with easy online access (http://dualrnaseq.molgenrug.nl). Further database exploration may expand our understanding of epithelial-pneumococcal interaction, leading to novel antimicrobial strategies.\n\nGraphical Abstract\n\nO_FIG O_LINKSMALLFIG WIDTH=199 HEIGHT=200 SRC=\"FIGDIR/small/048959_fig7.gif\" ALT=\"Figure 7\">\nView larger version (60K):\norg.highwire.dtl.DTLVardef@351367org.highwire.dtl.DTLVardef@8cd781org.highwire.dtl.DTLVardef@985c96org.highwire.dtl.DTLVardef@e6b6a0_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Rieza Aprianto, Jelle Slager, Siger Holsappel, Jan-Willem Veening. 2016-04-15. Time-resolved dual RNA-Seq reveals extensive rewiring of lung epithelial and pneumococcal transcriptomes during early infection. https://doi.org/10.1101/048959

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