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bioRxiv · 10.1101/047332

Nucleosome dynamics and maintenance of epigenetic states of CpG islands

Ian B Dodd·Kim Sneppen
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Abstract

Methylation in mammalian DNA occurs primarily at CpG sequences. The CpG sites are distributed in high density clusters (or islands) separated by extended regions of low density. Cluster methylation tends to be bimodal, being dominantly unmethylated or mostly methylated. For CpG clusters near promoters, low methylation is associated with transcriptional activity, while high methylation is associated with gene silencing. Alternative CpG methylation states are thought to be stable and heritable, conferring localized epigenetic memory that allows transient signals to create long-lived gene expression states. Positive feedback where methylated CpG sites recruit enzymes that methylate nearby CpGs, does not easily explain that as clusters increase in size or density they change from being primarily methylated to primarily unmethylated. Here, we show that an interaction between the methylation state of a cluster and its occupancy by nucleosomes provides a mechanism to reproduce epigenetic potential and the genome wide systematics of CpG islands.

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Ian B Dodd, Kim Sneppen. 2016-04-05. Nucleosome dynamics and maintenance of epigenetic states of CpG islands. https://doi.org/10.1101/047332

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