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bioRxiv · 10.1101/041525

Generation of mutation hotspots in ageing bacterial colonies

Agnieszka Sekowska·Sofie Wendel·Morten Helge Hauberg Norholm·Antoine Danchin
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Abstract

How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome and over time, mutations increasingly originated from 8-oxo-guanosine, formed exclusively on the transcribed strand.

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Agnieszka Sekowska, Sofie Wendel, Morten Helge Hauberg Norholm, Antoine Danchin. 2016-02-26. Generation of mutation hotspots in ageing bacterial colonies. https://doi.org/10.1101/041525

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