Ex vivo mycobacterial growth inhibition assay (MGIA) for tuberculosis vaccine testing - a protocol for mouse splenocytes

Andrea Andrea Zelmer; Rachel Tanner; Elena Stylianou; Sheldon Morris; Angelo Izzo; Ann Williams; Sally Sharpe; Ilaria Pepponi; Barry Walker; David A Hokey; Helen McShane; Michael Brennan; Helen Fletcher

doi:10.1101/020560

bioRxiv · 10.1101/020560

Ex vivo mycobacterial growth inhibition assay (MGIA) for tuberculosis vaccine testing - a protocol for mouse splenocytes

Andrea Andrea Zelmer·Rachel Tanner·Elena Stylianou·Sheldon Morris·Angelo Izzo·Ann Williams·Sally Sharpe·Ilaria Pepponi·Barry Walker·David A Hokey·Helen McShane·Michael Brennan·Helen Fletcher

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Abstract

The testing of vaccines for tuberculosis is costly and time-consuming, and dependent on preclinical animal challenge models and clinical trials. We have recently developed a mycobacterial growth inhibition assay (MGIA) to test vaccine efficacy ex vivo. This assay measures the summative effect of the host immune response and may serve as a novel tool to facilitate vaccine testing. It has generated much interest recently, and to facilitate technology transfer and reproducibility between laboratories, we here describe a detailed protocol for an ex vivo MGIA in mouse splenocytes.

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Andrea Andrea Zelmer, Rachel Tanner, Elena Stylianou, Sheldon Morris, Angelo Izzo, Ann Williams, Sally Sharpe, Ilaria Pepponi, Barry Walker, David A Hokey, Helen McShane, Michael Brennan, Helen Fletcher. 2015-06-07. Ex vivo mycobacterial growth inhibition assay (MGIA) for tuberculosis vaccine testing - a protocol for mouse splenocytes. https://doi.org/10.1101/020560

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