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bioRxiv · 10.1101/011320

Wide field-of-view, twin-region two-photon imaging across extended cortical networks

Jeffrey N. Stirman·Ikuko T. Smith·Michael W. Kudenov·Spencer L. Smith
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Abstract

We demonstrate a two-photon imaging system with corrected optics including a custom objective that provides cellular resolution across a 3.5 mm field of view (9.6 mm2). Temporally multiplexed excitation pathways can be independently repositioned in XY and Z to simultaneously image regions within the expanded field of view. We used this new imaging system to measure activity correlations between neurons in different cortical areas in awake mice.

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Jeffrey N. Stirman, Ikuko T. Smith, Michael W. Kudenov, Spencer L. Smith. 2014-11-12. Wide field-of-view, twin-region two-photon imaging across extended cortical networks. https://doi.org/10.1101/011320

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