TY - RPRT TI - When good guides go bad: empirical evaluation of all unique Cas9 targets in E. coli reveal widespread functionality and rules for gRNA biological design AU - Phillips, E. K. AU - Harrison, R. AU - Charles, S. AU - Klingeman, D. M. AU - Wiser, T. AU - Eckert, C. A. AU - Alexander, W. G. PY - 2025 DO - 10.1101/2025.05.23.651106 UR - https://www.biorxiv.org/content/10.1101/2025.05.23.651106v1 ID - 10.1101/2025.05.23.651106 ER -