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van der Wielen, N.

Publications and source records attributed to van der Wielen, N..

2 recordsLinked to original sources

Mycelium of mushroom-producing fungi as high-quality protein source

To achieve a more sustainable food system, reducing reliance on animal-based proteins is essential. Mycoprotein from mushroom-producing fungi (Basidiomycota) presents a promising yet underexplored alternative. Basidiomycetous mycelium combines the high protein quality of fungi with the sensory qualities of mushrooms. This study compares the mycelium of commercially cultivated and edible basidiomycetes to their fruiting bodies and to mycelium of Rhizopus microsporus var. oligosporus (tempeh fungus). Nutritional quality was assessed via protein content and PDCAAS, while sensory potential was analysed through equivalent umami concentration (EUC) and pyrazine levels. High-performing species surpassed the EUC of R. microsporus and fruiting bodies, reaching 301.0 g MSG-eq/100 g DW. Basidiomycetous mycelia exhibited superior protein quality (PDCAAS up to 1.20) and utilizable protein content (33.2 g/100 g DW), qualifying as an "Excellent protein source." Given the phylogenetic diversity of basidiomycetes, this study highlights the untapped potential of its mycelium as a high-quality protein source.

microbiology↗

An optimised approach to evaluate variability in gut health markers in healthy adults.

Despite advances in gut health research, the variability of important gut markers within individuals over time remains underexplored. We investigated the intra-individual variation of various faecal gut health markers using an optimised processing protocol aimed at reducing variability. Faecal samples from ten healthy adults over three consecutive days demonstrated marker-specific intra-individual coefficients of variation (CV%), namely: stool consistency (16.5%), water content (5.7%), pH (3.9%), total SCFAs (17.2%), total BCFAs (27.4%), total bacteria and fungi copies (40.6% and 66.7%), calprotectin and myeloperoxidase (63.8% and 106.5%), and untargeted metabolites (on average 40%). For thirteen microbiota genera, including Bifidobacterium and Akkermansia, variability exceeded 30%, whereas microbiota diversity was less variable (Phylogenetic Diversity 3.3%, Inverse Simpson 17.2%). Mill-homogenisation of frozen faeces significantly reduced the replicates CV% for total SCFAs (20.4% to 7.5%) and total BCFAs (15.9% to 7.8%), and untargeted metabolites compared to only faecal hammering, without altering mean concentrations. Our results show the potential need for repeated sampling to accurately represent specific gut health markers. We also demonstrated the effectiveness of optimised preprocessing of stool samples in reducing overall analytical variability.

microbiology↗