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van Himbeeck, R.

Publications and source records attributed to van Himbeeck, R..

3 recordsLinked to original sources

Fantastic Antagonists and Where to Find Them: The Global Distribution of Nematode-Antagonistic Fungi

Plant-parasitic nematodes can be suppressed by antagonistic members of the local soil microbiome. Compared to bacterial antagonists, considerably more attention has been paid to nematode-suppressing fungi. However, little is known about their global distribution. As their biogeography matters from both a fundamental and an applied perspective, we set out to mine the GlobalFungi database. After filtering on sample type, biome relevance and sequencing depth, we retained approximately 28,000 samples from 484 studies. An overview of fungal nematode antagonists was generated, and subsequent analyses revealed that 82.6% of the soil samples comprised [≥]1 nematode antagonist. Most of these antagonists are not obligatory nematode parasites; they switch between trophic lifestyles including saprophytism. Nematode antagonists are frequently found across most biomes; the detection probability was highest in croplands (88% of samples), woodlands (82%), and grasslands (76%). Most of the common nematode antagonists are present on all continents and across multiple climate zones underlining their enormous ecological flexibility. With one exception, the most frequently detected antagonistic fungi belonged to the fungal order Hypocreales whose members are known to parasitize insects and fungi as well. Analysis of the impact of temperature and precipitation on common antagonists in croplands revealed mostly non-linear responses. Among a selection of six soil properties, pH was the most informative predictor for the abundance of antagonists in croplands. Insights into the prevalence and the distribution of nematode antagonists at a global scale contribute to the exploration of the nematode-suppressive potential, which appears to be more common and widespread than often assumed.

ecology↗

Patchy distribution of potato cyst nematodes within single arable fields reveals local disease suppressiveness mediated by disparate microbialcommunities

Disease suppressiveness is a complex phenomenon that is assumed to be the resultant of actions of local microbial antagonists in soil environments. Exploitation of disease suppressiveness as a tool to manage pathogens is hindered by our poor understanding of this phenomenon. Here we investigated soil microbiome-based suppression of potato cyst nematodes (PCN), and, to this end, four apparently homogeneous potato fields with an unexplained non-homogeneous PCN distribution were selected. We hypothesized that this patchy PCN distribution resulted from local variation in disease suppressiveness. Under controlled greenhouse conditions, we confirmed the suppressiveness of these soils vis-a-vis PCN and soils were gamma-irradiated to corroborate the biotic origin of this suppression. Subsequent DNA-based analysis of the microbial community in the potato rhizosphere revealed suppressiveness-related contrasts in community composition between suppressive and conducive patches. Elevated abundances of fungal (e.g., Metacordyceps chlamydosporia) and bacterial (e.g., Pseudomonas fluorescens) nematode antagonists were positively correlated with PCN suppressive patches. Distinct sets of antagonists were found to be associated with PCN suppression despite of the geographical closeness of the locations under investigation. Our findings confirm the biotic origin of local PCN suppressiveness and reveal that it should be regarded as a superficially similar resultant of a biologically diverse phenomenon.

ecology↗

Disease-decreasing diversity: evidence for lowered parasite prevalence in microscopic nematode communities with higher species numbers

Biodiversity can affect parasite prevalence, a phenomenon widely studied in macroscopic organisms. However, data from microscopic communities is lacking, despite their essential role in ecosystem functioning and the unique experimental opportunities microscopic organisms offer. Here, we study diversity-disease effects in wild nematode communities by profiting from the molecular tools available in the well-studied model nematode Caenorhabditis elegans. Nanopore sequencing was used to characterize nematode community diversity and composition, whereas parasites were identified using nine distinct experimental assays based on fluorescent staining or fluorescent reporter strains. Our results indicate biotic stress is abundant in wild nematode communities. Moreover, in two assays, diversity-disease relations were observed: microsporidia and immune system activation were more often detected in relatively species-poor communities. Other assays, targeting different parasites, were without diversity-disease relations. Together, this study provides the first demonstration of diversity-disease effects in microbial communities and establishes the use of nematode communities as model systems for disease dilution.

ecology↗