Biology subjects

li, d.

Publications and source records attributed to li, d..

3 recordsLinked to original sources

bioRxiv2021

Molecular response of whitefly to the wild tomato Solanum habrochaites

The whitefly Bemisia tabaci (Gennadius) causes severe damage to cultivated tomato in many regions of the world through direct feeding and indirectly through transmission of plant viruses. Field observations show that B. tabaci is rarely infested the non-host plants such as the wild tomato Solanum habrochaites; however, the molecular mechanism involved in the recognition of wild plant odors is still unclear. In this study, we assessed the effects of S. habrochaites on the survival, fecundity, and egg hatchability of the Mediterranean (MED) species of B. tabaci. Expression and splicing of stress-response genes in whitefly exposed to S. habrochaites was analyzed using RNA-sequencing data and alternative splicing analysis. These results indicated that the S. habrochaites treatment can induce the expression of environmental stress genes in B. tabaci. This study may help us to a better understanding of the molecular mechanisms involved in the olfactory recognition of non-host volatiles particularly wild tomato relative. Furthermore, the findings of this study may provide excellent chances of finding a suitable antagonist of eco-friendly properties which can block the perception of chemosensory signals. Thereby, the feeding behavior and food preferences of B. tabaci can be manipulated and thus insect populations can eventually be controlled.

ecology↗

bioRxiv2020

A Reference Feature based method for Quantification and Identification of LC-MS based untargeted metabolomics

Batch inconsistency is a major problem when applying LC-MS based untargeted metabolomics in real-time analysis situation such as clinical diagnosis or health monitoring. And inefficiency of collecting MS2 is a major problem for metabolite identification. Here, we developed a reference-feature based quantification and identification strategy (RFQI). In RFQI, samples are individually profiled using a pre-fixed reference feature table. Quantification results show that RFQI improves features overlap rate and reduce variance across batches significantly in real-time-analysis mode, and can find more than 4-fold numbers of features. Besides, RFQI collects MS2 from consecutive increasing samples for metabolite identification of pre-fixed features, thus it can effectively compensate for the poor efficiency of MS2 collection in data-dependent acquisition mode. In summary, RFQI can make full advantage of consecutive increasing samples in real-time analysis situation, both for quantification and identification.

bioinformatics↗

bioRxiv2019

The Structural Basis for Glycerol Permeation by human AQP7

Human glycerol channel AQP7 conducts glycerol release from adipocyte and entry into the cells in pancreatic islets, muscles and kidney tubule, and thus regulate glycerol metabolism in those tissues. Compared with other human aquaglyceroporins, AQP7 shows a less conserved "NPA" motif in the center cavity, and a pair of aromatic residues at Ar/R selectivity filter. To understand the structural basis for the glycerol conductance, we crystallized the human AQP7 and determined the structure at 3.7 [A]. A substrate binding pocket was found near to the Ar/R filter and the bound glycerol molecule stabilized by R229. In vivo functional assay on human AQP7 as well as AQP3 and AQP10 demonstrated strong glycerol transportation activities at physiological condition. The human AQP7 structure reveals a fully closed conformation with its permeation pathway strictly confined by Ar/R filter at the exoplasmic side and the gate at the cytoplasmic side, and the dislocation of the residues at narrowest parts of glycerol pathway in AQP7 play a critical role in controlling the glycerol flux.

molecular biology↗