Biology subjects

Zolotukhin, S.

Publications and source records attributed to Zolotukhin, S..

3 recordsLinked to original sources

bioRxiv2022

Salivary Glands treated with AAV vectors encoding PYY and exendin-4 impact upon taste perception and body mass accumulation in mice.

The availability of high-calorie foods is likely a causative factor for high rates of obesity and metabolic disorders, which have been linked to food intake dysregulation. Several gut peptides have been implicated in feeding modulation and body mass accumulation. For example, glucagon peptide-like 1 (GLP-1) and peptide tyrosinetyrosine (PYY) have been shown to mediate satiety and reduce food intake. While systemic administration of such peptides has been explored as a therapy for metabolic disease, the effects of these hormones on taste signaling should also be considered given the importance of taste to feeding decisions. Peptide signaling systems are present in taste buds and oral GLP-1 and PYY signaling has been shown to influence taste responsiveness and feeding. Indeed, we previously demonstrated that genetic knockout of PYY in mice can impact on taste responsiveness and feeding and that viral overexpression of PYY in the salivary glands of these mice can rescue responsiveness. The present work uses AAV-mediated salivary gland treatment of both GLP-1 receptor agonist exendin-4 and PYY encoding vectors to explore the impact of the presence of these peptides on taste and body-mass accumulation in wild-type mice with intact peptide signaling systems. Results showed a significant effect of salivary gland treatment on responsiveness to multiple taste qualities. Treatment with a vector designed to overexpress both peptides in saliva resulted in substantial reduction in body mass accumulation. These findings show taste modulation and impacts on body mass accumulation by the targeting of salivary glands with vectors designed to overexpress metabolic peptides in wild-type mice and suggest that the taste bud is a promising substrate for food intake modulation.

neuroscience↗

bioRxiv2018

Taste Receptor Cells in Mice Express Receptors for the Hormone Adiponectin

The metabolic hormone adiponectin is secreted into the circulation by adipocytes, and mediates key biological functions including insulin sensitivity, adipocyte development, and fatty acid oxidation. Adiponectin is also abundant in saliva, where its functions are poorly understood. Here we report that murine taste receptor cells express adiponectin receptors, and may be a target for salivary adiponectin. Analysis of a transcriptome dataset obtained by RNA-seq analysis of purified circumvallate taste buds, revealed high expression levels for three adiponectin receptor types. Immunohistochemical studies showed that two of these receptors, AdipoR1 and T-cadherin, are localized to subsets of taste receptor cells. Immunofluorescence for T-cadherin was primarily co-localized with the Type 2 taste receptor cell marker phospholipase {beta}2, suggesting that adiponectin signaling could impact sweet, bitter, or umami taste signaling. However, adiponectin null mice showed no differences in taste responsiveness compared to wildtype controls in brief-access taste testing. AAV-mediated overexpression of adiponectin in the salivary glands of adiponectin null mice did result in a small but significant increase in behavioral taste responsiveness to the fat emulsion Intralipid. Together, these results suggest that salivary adiponectin can effect taste receptor cell function, though its impact on taste responsiveness and peripheral taste coding remains unclear.

animal behavior and cognition↗

bioRxiv2018

Differential Expression of Immune Related Genes in Taste Buds of Fed and Fasted Mice

To study the effects of feeding on taste receptor cell transcriptional regulation, we performed RNA-seq analysis of circumvallate taste buds isolated from mice before or after food consumption. Here we report and compare the taste bud transcriptomes obtained from food-deprived, satiated, and ad libitum fed control mice. Despite sample heterogeneity inherent to the whole taste bud transcriptome, bioinformatics analysis yielded 144 differentially expressed transcripts associated with immunity, cytoskeletal structure, and protein folding between these groups. We also profiled the transcriptome obtained from ad libitum fed control mice based on receptor related gene ontology terms, demonstrating a use of this dataset in the identification of novel TRC receptors. The data presented here suggest that transcriptional regulation of immune cytokine signaling occurs in TRCs shortly after meal consumption, though additional analysis is required to confirm this hypothesis.

bioinformatics↗