Biology subjects

Yong, S.

Publications and source records attributed to Yong, S..

3 recordsLinked to original sources

bioRxiv2026

High-resolution single-cell atlas of the human B cell compartment and immune microenvironment across tissues

The immune system is a dynamic network of diverse cell types distributed across tissues. While mouse studies have highlighted the importance of tissue-localized B-cell responses in infection and tissue repair, research on human B cells has remained largely confined to peripheral blood. Here, we specifically investigated the human B-cell compartment and its interactions with the immune microenvironment across 10 tissues, using single-cell RNA sequencing and paired B-cell receptor sequencing. We mapped the full spectrum of B-cell populations and revealed diverse differentiation trajectories spanning naive, memory, and plasma cell types. Germinal center B cells and plasma cells showed tissue-specific adaptations in transcriptional states, isotype usage, and functional profiles. Plasma cell isotypes influenced both effector functions and predicted interactions with T cells. Finally, we defined tissue-specific residency gene modules that outperformed existing memory B-cell signatures. Together, this dataset serves as a foundation for systematically studying tissue-localized B cells and reveals how local microenvironments shape humoral immunity.

immunology↗

bioRxiv2026

Toward Textile-Integrated Electrochemical Systems: A Flexible PCB Potentiostat for Wearable Glucose Monitoring

Flexible and textile-integrated electrochemical systems offer a convenient, user-friendly and non-invasive platform for continuous biochemical monitoring. In this study, a fully flexible and low-profile electrochemical system was developed by fabricating both the glucose biosensor and a compact potentiostat implemented on a polyimide (PI) filament circuit. The glucose biosensor was realized via direct laser writing (DLW), enabling precise electrode patterning and seamless integration with the potentiostat filament circuit. The integrated system exhibited a linear chronoamperometric response to glucose concentrations ranging from 0 to 0.25 mM in artificial sweat (AS). Further evaluation on cotton textiles soaked in AS and under mechanical bending confirmed stable performance, flexibility, and robustness. These findings highlight the potential of the PI-based potentiostat-sensor system for wearable, textile-integrated glucose monitoring and broader healthcare applications.

biochemistry↗

bioRxiv2022

Kinship and genetic variation in aquarium-spawned Acropora hyacinthus corals

Recent scientific advances in ex situ system design and operation make it possible to complete gametogenic cycles of broadcast spawning corals. Breeding corals in aquaria are critical advances for population management, particularly genetic rescue and assisted gene flow efforts. Genetic rescue projects for corals are already underway to bring threatened species into ex situ culture and propagation, thereby preserving standing genetic variation. However, while breeding corals is increasingly feasible, the consequences of the aquarium environment on the genetic and phenotypic composition of coral populations is not yet known. The aquarium environment may in itself be a selective pressure on corals, but it also presents relaxed selective pressure in other respects. In 2019 and 2020, gravid Acropora hyacinthus coral colonies were collected from Palauan reefs and shipped to the California Academy of Sciences (CAS) in San Francisco. In both years, gametes were batch-fertilized to produce larvae that were then settled and reared to recruits. As of April 2021, when they were sampled for sequencing, 23 corals produced at CAS in 2019 and 16 corals produced at CAS in 2020 had survived for two years and one year, respectively. We sequenced the full genomes of the 39 offspring corals and their 15 potential parents to a median 26x depth of coverage. We find clear differential parentage, with some parents producing the vast majority of offspring, while the majority of parents produced no surviving offspring. After scanning 12.9 million single nucleotide polymorphisms (SNPs), we found 887 SNPs that may be under selection in the aquarium environment, and we identified the genes and pathways these SNPs may affect. We present recommendations for preserving standing genetic variation in aquarium-bred corals based on the results of this pilot project.

evolutionary biology↗