Establishment of Integration-Free iPSCs from Diverse Porcine Species: A Novel Resource for Conservation and African Swine Fever Research
The accelerating biodiversity crisis, compounded by emerging infectious diseases like African swine fever (ASF), necessitate innovative conservation and disease management. ASF susceptibility varies wildly across species, from near-100% mortality in Asian suids to asymptomatic carriage in African forest species. We report the first successful derivation of integration-free induced pluripotent stem cells (iPSCs) from four phylogenetically distinct species: wild boar (Sus scrofa), Bornean bearded pig (Sus barbatus), Babirusa (Babyrousa babyrussa), and Red river hog (Potamochoerus porcus). Using Sendai virus-mediated reprogramming, we achieved efficiencies between 0.003% and 0.26%. These iPSCs were successfully differentiated into CD14CD11b monocytes - the primary target cells for the ASF virus - establishing a renewable, comparative research platform. This system enables host-pathogen studies previously hindered by ethical and logistical constraints of wildlife sampling. Beyond disease research, these iPSC lines serve as vital genetic repositories for endangered suids. Our methodology provides a replicable framework for extending stem cell technology to other conservation-priority taxa, demonstrating how high-tech cellular tools can advance both fundamental research and biodiversity preservation against emerging pathogen threats.