Biology subjects

Yellaboina, S.

Publications and source records attributed to Yellaboina, S..

3 recordsLinked to original sources

bioRxiv2025

MicroRNA-mRNA Gene Regulatory Circuitry Orchestrates the Epigenetic and Transcriptional Reprogramming from Pluripotency to Murine Mammary Epithelial stem-like-Cells lactogenic differentiation

MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression via mRNA degradation or translational repression. While miRNAs are established regulators of development and differentiation, their role in the dynamic mRNA transitions during lactogenic differentiation of mammary epithelial cells remains incompletely characterized. In this study, we profiled miRNA expression across four distinct developmental states: embryonic stem cells (ESCs), and three progressive stages of HC11 murine mammary epithelial differentiation, including undifferentiated growth factor-maintained cells (HC11-N), glucocorticoid-primed cells (HC11-P), and prolactin-induced lactogenic cells (HC11-PRL). By integrating these signatures with parallel mRNA expression profiles, we identified stage-specific miRNA-mRNA regulatory axes. Our analysis revealed miRNAs coordinate networks of transcription factors and epigenetic regulators to shape the lactogenic transcriptome. While ESCs and undifferentiated HC11 cells were characterized by pathways associated with pluripotency and oncogenic signalling, the pregnancy-like priming stage showed enrichment in, neurotrophin signalling. Crucially, the lactation-like state was dominated by PI3K-Akt and mTOR signalling-- critical drivers of epithelial proliferation and milk synthesis. Furthermore, the integration of miRNAs with transcriptional and epigenetic regulators highlighted the modulation of prolactin signalling and extracellular matrix-receptor interactions during lactation. This study provides a comprehensive map of the miRNA-associated post-transcriptional landscape of lactogenesis, offering a mechanistic framework for understanding mammary gland development and associated pathologies.

genomics↗

bioRxiv2018

The Cuon Enigma: Genome survey and comparative genomics of the endangered Dhole (Cuon alpinus)

The Asiatic wild dog is an endangered monophyletic canid restricted to Asia; facing threats from habitat fragmentation and other anthropogenic factors. Dholes have unique adaptations as compared to other wolf-like canids for large litter size (larger number of mammae) and hypercarnivory making it evolutionarily notable. Over evolutionary time, dhole and the subsequent divergent wild canids have lost coat patterns found in African wild dog. Here we report the first high coverage genome survey of Asiatic wild dog and mapped it with African wild dog, dingo and domestic dog to assess the structural variants. We generated a total of 124.8 Gb data from 416140921 raw read pairs and retained 398659457 reads with 52X coverage and mapped 99.16% of the clean reads to the three reference genomes. We identified ~13553269 SNVs, ~2858184 InDels, ~41000 SVs, ~1854109 SSRs and about 1000 CNVs. We compared the annotated genome of dingo and domestic dog with dhole genome sequence to understand the role of genes responsible in pelage pattern, dentition and mammary glands. Positively selected genes for these phenotypes were looked for SNP variants and top ranked genes for coat pattern, dentition and mammary glands were found to play a role in signalling and developmental pathways. Mitochondrial genome assembly predicted 35 genes, 11 CDS and 24 tRNA. This genome information will help in understanding the divergence of two monophlyletic canids, Cuon and Lycaon, and the evolutionary adaptations of dholes with respect to other canids.

genomics↗

bioRxiv2017

Polymorphic Dynamics of Ribosomal Proteins′ Gene Expression during Induced Pluripotency

Factor induced pluripotent stem cells (iPSCs) offer great promise in regenerative medicine. However, accumulating evidence suggests that iPSCs are heterogeneous in comparison with embryonic stem cells (ESCs), and that is attributed to various genetic and epigenetic states of donor cells. In the light of the discovery of cell-type specialized ribosomal protein composition, its role as the cells transit through different stages of reprogramming and when iPSCs differentiate into specialized cell-types has not been explored to understand its influence in the reprogramming and differentiation process and outcome. By re-analyzing the publicly available gene expression datasets among ESCs, various sources of iPSCs and somatic cells and by studying the ribosomal protein gene expression during different stages of reprogramming of somatic cells and different passages of established iPSCs we found distinct patterns of their expression across multiple cell-types. We experimentally validated these results on the cells undergoing reprogramming from human dermal fibroblasts. Finally, by comparing publicly available data from iPSCs, iPSCs derived specialized cells and its in vivo counterparts, we show alterations in ribosomal gene expression during differentiation of specialized cells from iPSCs which may have Implications in the context of ribosomopathies. Our results provide an informatics framework for researchers in efficient generation of iPSCs that are equivalent to ESCs.

developmental biology↗