Biology subjects

Yaguzhinsky, L. S.

Publications and source records attributed to Yaguzhinsky, L. S..

2 recordsLinked to original sources

bioRxiv2020

Oligomeric hypercompex of the complete oxidative phosphorylation system in heart mitochondria.

The existence of a complete oxidative phosphorylation system supercomplex including both electron transport system and ATP synthases has long been assumed based on functional evidence. However, no conclusive structural confirmation has been obtained. In this study cryo-electron tomography was used to reveal the supramolecular architecture of the rat heart mitochondria cristae. We show that rows of respiratory chain supercomplexes are connected with rows of ATP synthases forming the oligomeric hypercomplex. The discovered hypercomplexes may increase the effectiveness of oxidative phosphorylation ensuring a direct proton transfer from pumps to ATP synthase along the membrane without energy dissipation.

molecular biology↗

bioRxiv2020

Toxic effect of 2,2'-bis(bicyclo heptane) on bacterial cells.

Here we present the study of the genotoxic effect of a 2,2-bis(bicyclo[2.2.1]heptane) (BBH), which is promising as a fuel component for liquid rocket engines. The use of Escherichia coli lux-biosensors showed that in addition to DNA damage causing SOS-response, there is also an oxidative effect on cells. The greatest toxicity is determined by the mechanism of formation of superoxide anion radical and is detected by the lux biosensor E. coli pSoxS-lux, in which the genes of bacterial luciferases are transcriptionally crosslinked with the promoter of the soxS gene. It is assumed that the oxidation of BBH leads to the formation of reactive oxygen species, which should give the main contribution to the toxicity of this substance.

pharmacology and toxicology↗