Biology subjects

Wong, C.-W.

Publications and source records attributed to Wong, C.-W..

3 recordsLinked to original sources

bioRxiv2023

Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrow

Ectopic bone marrow adipocytes (BMAds) accumulation occurring under diverse pathophysiological conditions leads to bone deterioration. Estrogen-related receptor (ESRRA) is a key regulator responding to metabolic stress. Here, we show that adipocyte-specific ESRRA deficiency rescues osteogenesis and vascular formation in adipocyte-rich bone marrow due to estrogen deficiency or obesity. Mechanistically, adipocyte ESRRA interferes with E2/ESR1 signaling resulting in transcriptional repression of secreted phosphoprotein 1 (Spp1); and positively modulates Leptin expression by binding to its promoter. ESRRA abrogation results in enhanced SPP1 and decreased LEPTIN secretion from both visceral adipocytes and BMAds, concertedly dictating bone marrow stromal stem cell fate commitment and restoring type H vessel formation, constituting a feed-forward loop for bone formation. Pharmacological inhibition of ESRRA protects obese mice against bone loss and high marrow adiposity. Thus, our findings highlight a therapeutic approach via targeting adipocyte ESRRA to preserve bone formation especially in detrimental adipocyte-rich bone milieu. Graphic abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=113 SRC="FIGDIR/small/552932v1_ufig1.gif" ALT="Figure 1"> View larger version (33K): org.highwire.dtl.DTLVardef@1132da3org.highwire.dtl.DTLVardef@19342aorg.highwire.dtl.DTLVardef@afe8d7org.highwire.dtl.DTLVardef@dfb1f9_HPS_FORMAT_FIGEXP M_FIG C_FIG

molecular biology↗

bioRxiv2021

Cell transcriptomic atlas of the non-human primate Macaca fascicularis

Studying tissue composition and function in non-human primates (NHP) is crucial to understand the nature of our own species. Here, we present a large-scale single-cell and single-nucleus transcriptomic atlas encompassing over one million cells from 43 tissues from the adult NHP Macaca fascicularis. This dataset provides a vast, carefully annotated, resource to study a species phylogenetically close to humans. As proof of principle, we have reconstructed the cell-cell interaction networks driving Wnt signalling across the body, mapped the distribution of receptors and co-receptors for viruses causing human infectious diseases and intersected our data with human genetic disease orthologous coordinates to identify both expected and unexpected associations. Our Macaca fascicularis cell atlas constitutes an essential reference for future single-cell studies in human and NHP.

cell biology↗

bioRxiv2019

Learning Your Heart Actions From Pulse: ECG Waveform Reconstruction From PPG

This paper studies the relation between electrocardiogram (ECG) and photoplethysmogram (PPG) and infers the waveform of ECG via the PPG signals that can be obtained from affordable wearable Internet-of-Things (IoT) devices for mobile health. In order to address this inverse problem, a transform is proposed to map the discrete cosine transform (DCT) coefficients of each PPG cycle to those of the corresponding ECG cycle based on our proposed cardiovascular signal model. The proposed method is evaluated with different morphologies of the PPG and ECG signals on three benchmark datasets with a variety of combinations of age, weight, and health conditions using different training setups. Experimental results show that the proposed method can achieve a high prediction accuracy greater than 0.92 in averaged correlation for each dataset when the model is trained subject-wise. With a signal processing and learning system that is designed synergistically, we are able to reconstruct ECG signals by exploiting the relation of these two types of cardiovascular measurement. The reconstruction capability of the proposed method can enable low-cost ECG screening from affordable wearable IoT devices for continuous and long-term monitoring. This work may open up a new research direction to transfer the understanding of the clinical ECG knowledge base to build a knowledge base for PPG and data from wearable devices.

bioinformatics↗