Waymire, E. | bioRxiv Science

bioRxiv2025

Genetic surveillance of Plasmodium-Anopheles compatibility markers during Anopheles stephensi associated malaria outbreak

Despite previous decline of malaria in Ethiopia, an outbreak in Dire Dawa in 2022 implicated invasive vector An. stephensi as responsible. The transmission of Plasmodium by invasive An. stephensi raises questions about the molecular basis of compatibility, and the origin of the Plasmodium being transmitted. The Plasmodium P47 gene is a parasite-vector interaction gene in Anopheles, and along with corresponding mosquito P47 receptor (P47Rec), can be critical in establishment of Plasmodium infections in anophelines. Here, we analyzed P47 and P47Rec sequences to determine the origin of Plasmodium detected in An. stephensi during the outbreak and evaluate markers of compatibility. Analysis of geographically informative SNPs in Pfs47 revealed that these P. falciparum exhibit the African haplotype. We also identified a single amino acid change in P47Rec within these An. stephensi, which could act as a marker for the propensity of An. stephensi populations to outbreaks. Together, we provide the basis for further study to deepen the understanding of invasive An. stephensi -African Plasmodium interactions to better control transmission of malaria and prevent further outbreaks.

evolutionary biology↗

bioRxiv2023

Building the vector in? Construction practices contribute to the invasion and persistence of Anopheles stephensi in Jigjiga, Ethiopia

Anopheles stephensi is a major vector of malaria in Asia and the Arabian Peninsula, and its recent invasion into Africa poses a significant threat to malaria control and elimination efforts on the continent. The mosquito is well-adapted to urban environments, and its presence in Africa could potentially lead to an increase in malaria transmission in cities. Most of the knowledge about An. stephensi ecology in Africa has been generated from studies conducted during the rainy season, when vectors are most abundant. Here, we provide evidence from the peak of the dry season in the city of Jigjiga, Ethiopia, and report the finding of An. stephensi immature stages infesting predominantly water reservoirs made to support construction operations (in construction sites or associated with brick manufacturing businesses). Political and economic changes in Ethiopia (and particularly the Somali Region) have fueled an unprecedented construction boom since 2018 that, in our opinion, has been instrumental in the establishment, persistence and propagation of An. stephensi via the year-round availability of perennial larval habitats associated with construction. We argue that larval source management during the dry season may provide a unique opportunity for focused control of An. stephensi in Jigjiga and similar areas.

ecology↗