Biology subjects

Watsa, M.

Publications and source records attributed to Watsa, M..

4 recordsLinked to original sources

bioRxiv2026

Multiomic evidence for microbial contributions to species-specific olfactory communication in wild tamarins

Closely related sympatric species are hypothesized to face strong selection against hybridization, favoring species-specific signals that maintain reproductive isolation. Within species, olfaction may also mediate social interactions and facilitate social cohesion. Here, we investigated the potential for microbiome-derived scent signals to contribute to inter- and intra-species communication in two sympatric wild tamarins (Leontocebus weddelli and Tamarinus subgrisescens). We analyzed scent gland samples to characterize odor profiles (volatile organic compounds, VOCs) and microbiome taxonomic composition and putative functions from metagenomic shotgun sequences. Species membership was predicted accurately from microbiome profiles, whereas VOCs alone discriminated poorly between species. We found high abundance of Corynebacterium in L. weddelli, a genus associated with fatty acid metabolism, alongside the exclusive detection of acetic acid, highlighting microbially derived fatty acids as candidates for further investigation in this species. In T. subgrisescens, we found a more diverse microbiota, with fewer taxa known to be related to odor production, while VOC profiles appeared less variable among individuals. In contrast, group-level signatures were weak, suggesting that intra-specific odor may not strongly contribute to signaling group membership. We highlight the challenge of linking high-dimensional microbial communities, which include taxa not necessarily involved in odor production, to the smaller dataset of detectable VOCs. Although scent gland microbiota differed between tamarin species, whether these differences translate into species-specific odor cues remains to be tested. Future research involving behavioural assays and targeted experimental approaches that focus on key microbes and metabolites may shed further insight into roles and mechanisms of olfactory communication.

evolutionary biology↗

bioRxiv2019

Genomics In The Jungle: Using Portable Sequencing As A Teaching Tool In Field Courses

Genetic research is a rapidly evolving field of study that is increasingly being utilized as a tool for wildlife conservation. However, researchers and science educators in remote areas can often find it difficult to access the latest genetic technologies, often due to a combination of high costs, bulky equipment, and lack of infrastructure. Recent technological innovations are resulting in portable, low-cost instruments that enable next-generation sequencing in remote environments, offering new opportunities to generate a more widespread network of trained conservation scientists, particularly within regions of high biodiversity. What is currently lacking are formalized educational efforts to teach participants in biodiverse areas with hands-on training in molecular biology and real-time DNA sequencing techniques. To address this challenge, we report the design and summarized feedback/outcomes of a conservation genetics field course, called Genomics in the Jungle, that took place at a field research station in the Amazon rainforest of southeastern Peru. The program was established by a small US-based NGO, Field Projects International, and facilitated by a local eco-tourism company in Peru, Inkaterra. We utilized portable sequencing technologies from Oxford Nanopore Technologies, and in-kind support from the manufacturers MiniPCR, MiniOne Systems, Promega, and New England Biolabs. Participants included a mix of non-Peruvian students and local/regional students, some of which had no prior exposure to a genetics laboratory. Overall, we maintain that portable sequencing technology is democratizing scientific research and conservation efforts, and is a major step forward for science educators and conservationists.

scientific communication and education↗

bioRxiv2019

Vocal Transmission of Breeding Status May Facilitate Dispersal in a Cooperative Breeding Primate

Social complexity may drive complexity in communicative systems due to an individual's need to navigate unpredictable interactions with multiple conspecifics. Cooperative breeding primates (marmosets and tamarins; family: Callitrichidae) live in groups with moderate to high reproductive skew, particularly in females, whereby sexually mature individuals are frequently prevented from breeding. Remarkably, dispersal from natal groups is not stereotyped upon reaching reproductive maturity. Individuals are often observed remaining in their natal groups until the same-sex breeder in their group or a neighboring group dies, experiencing hormonal reproductive suppression, aggression, and limited access to potential mates. Here we examined whether emperor tamarins (Saguinus imperator) might use vocal signals to reduce dispersal risks and maximize the likelihood of attaining a breeding position. Using six consecutive years of mark-recapture data, we showed that sexually mature non-breeders (herein \"secondary breeders\") are more likely to leave their groups from one year to the next than sexually mature breeders (\"primary breeders\"). This confirmed that, unlike primary breeders who do not need to disperse in order to reproduce, secondary breeders are choosing to accept the risks associated with dispersal and emigrating from their natal groups. We used neural networks to classify vocalizations according to individual breeding status, and conducted a series of playback experiments which demonstrated that tamarins discriminated between the calls of primary and secondary breeders. Our data support the hypotheses that secondary breeders disperse to increase mating opportunities and use vocalizations to signal their availability to potential mates. This species of cooperative breeder appears to use vocalization to navigate its social and reproductive systems, minimizing risks of dispersal and in turn increasing the likelihood of reproductive success. This research has important implications for our understanding of sexual signaling, partner choice, and reproductive success in cooperative breeders.

animal behavior and cognition↗

bioRxiv2018

A multi-year survey of helminths from the gastrointestinal tract of wild saddleback (Leontocebus weddelli) and emperor (Saguinus imperator) tamarins

Noninvasive monitoring of gastrointestinal parasites from wild primates demonstrates that parasite-host relationships are altered during habitat or climatic disturbances. Interpreting changes in parasite measures for population health monitoring is problematic, since wild primates are infected with multiple parasites that fluctuate temporally and seasonally. Individual parasite infection data from two wild populations of New World primates, the saddleback (Leontocebus weddelli) and emperor (Saguinus imperator) tamarin, were collected over three years to: 1) establish baseline levels of parasite species richness (PSR) and variation across demography; 2) test for non-random associations of parasite co-occurrence; and 3) test hypothesized relationships between group size and PSR. Ten distinguishable parasite taxa were identified from 288 fecal samples by light microscopy following centrifugation and ethyl-acetate sedimentation. These samples represented 105 unique individuals (71 saddleback and 34 emperor tamarins), across 13 saddleback and seven emperor groups. Of the parasites identified in this study, none were confirmed as host specific, and only two parasites had statistically different prevalence between the host species. With few exceptions, individual infection status remained relatively unchanged over the study period. Considering yearly pair-wise parasite associations, we detected no marked differences between expected and observed levels of co-infection, nor did we detect statistically significant associations between group size and parasite species richness over 30 group-years. Logistic models of individual infection status did not identify a sex bias; however, age or species predicted the presence of four and three parasite taxa, respectively. Our model found higher PSR for saddleback tamarins. Considering the two most common parasites, one is typically pathogenic and the other is not, reinforcing caution when translating clinical findings of pathology to real-world systems. We now have reliable baseline data for future monitoring of these populations. Next steps should involve the molecular characterization of these parasites, and the exploration of linkages with health parameters.

ecology↗