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Viergever, B. J.

Publications and source records attributed to Viergever, B. J..

2 recordsLinked to original sources

ATR inhibitors synergize with mitomycin C to enhance cytotoxicity in patient-derived non-muscle invasive bladder cancer organoids

Background and ObjectiveThere is a high recurrence rate in non-muscle invasive bladder cancer (NMIBC) patients treated with intravesical chemotherapy or BCG, indicating the need for novel treatment options. Synergy between DNA damage response inhibitors and chemotherapy has been shown for different solid tumors. In this study, we explore whether combining chemotherapeutic agents with Ataxia telangiectasia and Rad3-related (ATR) kinase inhibitors is a suitable strategy to reduce recurrence in NMIBC. MethodsNMIBC patient-derived organoids (PDOs; n=6) were exposed to mitomycin C (MMC), gemcitabine, or epirubicin for 2h, to mimic intravesical instillation, and subsequently to ATR inhibitors (berzosertib, ceralasertib, tuvusertib) for 72h. Cell viability and PDO regrowth potential was determined by microscopy and CellTiter-Glo(R) luminescence assays, during a period of 6 weeks post-treatment. Key Findings and LimitationsPDO viability was severely impaired (1-22% viable cells at t=6 weeks) after combination treatment with MMC and ATR inhibitors. This effect was observed directly after treatment and maintained during 6 weeks after drug exposure. In contrast, PDOs treated with only chemotherapy or ATR inhibitors showed similar proliferation rates as untreated controls in week 6. LimitationsPDO models were all chemotherapy naive (either BCG or no pre-treatment). It would be interesting to confirm these findings in MMC-resistant models. Conclusions and Clinical ImplicationsThis study demonstrates synergy between different ATR inhibitors and MMC in a panel of six NMIBC PDOs. We propose that combining intravesical chemotherapy instillations with DNA damage response inhibitors should be further evaluated as a promising strategy to reduce recurrence rates for NMIBC. Plain English summaryIn this report we looked at new treatment options for non-muscle invasive bladder cancer. We discovered that tumor cells are killed very efficiently if chemotherapy is combined with drugs that prevent cells from repairing DNA damage.

cancer biology↗

Organization and dynamics of the cortical complexes controlling insulin secretion in β-cells.

Insulin secretion in pancreatic {beta}-cells is regulated by cortical complexes that are enriched at the sites of adhesion to extracellular matrix facing the vasculature. Many components of these complexes, including Bassoon, RIM, ELKS and liprins, are shared with neuronal synapses. Here, we show that insulin secretion sites also contain non-neuronal proteins LL5{beta} and KANK1, which in migrating cells organize exocytotic machinery in the vicinity of integrin-based adhesions. Depletion of LL5{beta} or focal adhesion disassembly triggered by myosin II inhibition perturbed the clustering of secretory complexes and attenuated the first wave of insulin release. While previous analyses in vitro and in neurons suggested that secretory machinery might assemble through liquid-liquid phase separation, analysis of endogenously labeled ELKS in pancreatic islets indicated that its dynamics is inconsistent with such a scenario. Instead, fluorescence recovery after photobleaching and single molecule imaging showed that ELKS turnover is driven by binding and unbinding to low-mobility scaffolds. Both the scaffold movements and ELKS exchange were stimulated by glucose treatment. Our findings help to explain how integrin-based adhesions control spatial organization of glucose-stimulated insulin release.

cell biology↗