Biology subjects

Vernier, P.

Publications and source records attributed to Vernier, P..

2 recordsLinked to original sources

bioRxiv2021

Plasticity of the dopaminergic phenotype and of locomotion in larval zebrafish induced by changes in brain excitability during the embryonic period.

During the embryonic period, neuronal communication starts before the establishment of the synapses with alternative forms of neuronal excitability, called here Embryonic Neuronal Excitability (ENE). ENE has been shown to modulate the unfolding of development transcriptional programs but the global consequences for the developing organisms are not all understood. Here we monitored calcium transients in zebrafish embryos as a proxy for ENE to assess the efficacy of transient pharmacological treatments to either increase or decrease ENE. Increasing or decreasing ENE for 24 hours at 2 days post fertilization (dpf), at the end of the embryonic period, promoted respectively an increase or a decrease in the numbers of dopamine (DA) neurons in the telencephalon and in the olfactory bulb of zebrafish larvae at 6 dpf. This plasticity of dopaminergic specification occurs within a stable population of vMAT2-positive cells, hence identifying an unanticipated biological marker for this reserve pool of of DA neurons that can be recruited by increasing ENE. Modulating ENE also affected larval locomotion several days after the end of the treatments. In particular, the increase of ENE from 2 to 3 dpf promoted hyperlocomotion of larvae at 6 dpf, reminiscent of endophenotypes reported for Attention Deficit with Hyperactivity Disorders and schizophrenia in zebrafish. These results provide a convenient framework to identify environmental factors that could disturb ENE as well as to study the molecular mechanisms linking ENE to neurotransmitter specification, with relevance to the pathogenesis of neurodevelopmental disorders. Significance Statement- Spontaneous calcium transients, used as a proxy for Embryonic Neuronal Excitability (ENE), are detected in the forebrain of embryonic zebrafish. - Short-term pharmacological treatments by bath application could increase or decrease ENE. - The post-mitotic differentiation of the dopaminergic phenotype is modulated by ENE in the zebrafish forebrain. - The plasticity of the dopaminergic specification occurs within a reserve pool of vMAT2-positive cells. - Transient increase of ENE at the end of the embryonic period induces hyperlocomotion, a phenotype associated with ADHD and schizophrenia in this model. - Our results open clinically relevant perspectives to study the pathogenesis of neurodevelopmental disorders in zebrafish.

neuroscience↗

bioRxiv2020

Comparing global and regional maps of intactness in the boreal region of North America: Implications for conservation planning in one of the world's remaining wilderness areas

Though North Americas boreal forest contains some of the largest remaining intact and wild ecosystems in the world, human activities are systematically reducing its extent. Consequently, forest intactness and human influence maps are increasingly used for monitoring and conservation planning in the boreal region. We compare eight forest intactness and human impact maps to provide a multi-model assessment of intactness in the boreal region. All maps are global in extent except for Global Forest Watch Canadas Human Access (2000) and Intact Forest Landscapes (2000, 2013) maps, although some global maps are restricted to areas that were at least 20% treed. As a function of each maps spatial coverage in North America, the area identified as intact ranged from 55% to 79% in Canada and from 32% to 96% in Alaska. Likewise, the similarity between pairs of datasets in the Canadian boreal ranged from 0.58 to 0.86 on a scale of 0-1. In total, 45% of the region was identified as intact by the seven most recent datasets. There was also variation in the ability of the datasets to account for anthropogenic disturbances that are increasingly common in the boreal region, such as those associated with resource extraction. In comparison to a recently developed high resolution regional disturbance dataset, the four human influence datasets (Human Footprint, Global Human Modification, Large Intact Areas, and Anthropogenic Biomes), in particular, omitted 59-85% of all linear disturbances and 54-89% of all polygonal disturbances. In contrast, the global IFL, Canadian IFL, and Human Access maps omitted 2-7% of linear disturbances and 0.1-5% of polygonal disturbances. Several differences in map characteristics, including input datasets and methods used to develop the maps may help explain these differences. Ultimately, the decision on which dataset to use will depend on the objectives of each specific conservation planning project, but we recommend using datasets that 1) incorporate regional anthropogenic activities, 2) are updated regularly, 3) provide detailed information of the methods and input data used, and 4) can be replicated and adapted for local use. This is especially important in landscapes that are undergoing rapid change due to development, such as the boreal forest of North America.

ecology↗