Biology subjects

Venkatesan, P.

Publications and source records attributed to Venkatesan, P..

3 recordsLinked to original sources

bioRxiv2025

Comprehensive Genotoxicity and Subchronic Oral Toxicity Evaluation of Kumamolisin from Bacillus sp. MN-32 for Human Use

Kumamolisin from Bacillus sp. MN-32 (produced by E. coli B/BL21 background) is an acid-active sedolisin protease developed for food uses; its systemic safety was assessed using GLP (Good-laboratory-practice) in vitro genotoxicity assays and repeated-dose oral studies. A bacterial reverse-mutation assay (OECD TG 471; Salmonella TA98, TA100, TA102, TA1535, TA1537; plate-incorporation and preincubation; {+/-}S9) showed no concentration-related increases in revertants up to 5000 {micro}g total organic solids (TOS)/plate. A mammalian cell micronucleus test (OECD TG 487; human lymphocytes; 4 h {+/-}S9 and 24 h -S9) showed no significant increases in micronucleated binucleated cells at 1250-5000 {micro}g TOS/mL, with cytotoxicity [≤] 21.6% by CBPI (Cytokinesis-Block Proliferation Index). In 28-day and 90-day repeated dose toxicity studies Sprague Dawley rat studies (OECD TG 407/408; n = 10/sex/group) at 0, 500, 1000, and 2000 mg TOS/kg bw/day by gavage (10 mL/kg/day), survival was 100% and in-life observations, ophthalmology, neurobehavior, urinalysis, hematology, coagulation, and clinical chemistry were unremarkable; isolated statistical differences (e.g., male total protein, BUN, glucose, LDL; calcium; female phosphorus) were marginal, within historical ranges, non-monotonic, and lacked histopathology correlates. Organ-weight differences were single-sex and non-dose-dependent. No test-item-related lesions were detected. The NOAEL was 2000 mg TOS/kg bw/day ((highest dose tested) in both the 28-day and 90-day studies, supporting oral safety for food-use exposure.

pharmacology and toxicology↗

bioRxiv2024

Systemic inflammation and lymphocyte activation precede rheumatoid arthritis

Some autoimmune diseases, including rheumatoid arthritis (RA), are preceded by a critical subclinical phase of disease activity. Proactive clinical management is hampered by a lack of biological understanding of this subclinical at-risk state and the changes underlying disease development. In a cross-sectional and longitudinal multi-omics study of peripheral immunity in the autoantibody-positive at-risk for RA period, we identified systemic inflammation, proinflammatory-skewed B cells, expanded Tfh17-like cells, epigenetic bias in naive T cells, TNF+IL1B+ monocytes resembling a synovial macrophage population, and CD4 T cell transcriptional features resembling those suppressed by abatacept (CTLA4-Ig) in RA patients. Our findings characterize pathogenesis prior to clinical diagnosis and suggest the at-risk state exhibits substantial immune alterations that could potentially be targeted for early intervention to delay or prevent autoimmunity. We provide a suite of tools at https://apps.allenimmunology.org/aifi/insights/ra-progression/ to facilitate exploration and enhance accessibility of this extensive dataset. One Sentence SummaryACPA+ at-risk individuals show RA-like inflammation and multi-compartment immune dysregulation during transition to clinically active RA

immunology↗

bioRxiv2018

STUDY ON THE INFLAMMATORY RESPONSE OF PMMA/POLYSTYRENE/SILICA NANOCOMPOSITE MEMBRANES FOR DRUG DELIVERY AND DENTAL APPLICATIONS

BackgroundThe application of polymeric materials in medical industry has grown drastically in the last two decades due to their various advantages compared to existing materials. The present research work emphases on the sol-gel technique to formulate the polymethyl methyl acrylate/polystyrene/silica composite membrane.\n\nMethodsThe characteristic of the composite was investigated through modern state art of instrumentation.\n\nResultsThe functional groups attached to the polymer was absorbed by FTIR. The FTIR spectrum confirm that the blend was mixed thoroughly and the formation of unite intimately between the polymers. The membranes were observed by SEM for its surface homogeneity which depends upon the composition of the two blending polymers. The captured SEM images showed the formation of microcracks on the surface, which was evidently controlled by varying the constituent polymer ratios. The prepared blend membranes with 2:1 ratio of PMMA/PS/Si displayed higher water uptake compared to other blended membranes. The composite membranes had good hydroxyl apatite growth in SBF solution. Furthermore, the in vitro cytotoxicity studies carried out by MTT method, using RAW macrophage cells showed that all the samples exhibited excellent cell viability.\n\nConclusionThe inflammatory response of composite with equal concentration of PMMA-PS were performed and observed no inflammation in comparison with control and other tested concentrations.

scientific communication and education↗