Biology subjects

Tsuchiya, H.

Publications and source records attributed to Tsuchiya, H..

3 recordsLinked to original sources

bioRxiv2019

Autologous adipose-derived stem cell transplantation enhances healing of wound with exposed bone in a rat model

ObjectivesSoft tissue wounds with exposed bone often require extended healing times and can be associated with severe complications. We describe the ability of artificial dermis with autogenic adipose-derived stem cells (ADSCs) to promote the healing of wounds with exposed bone in a rat model.\n\nMethodsAdipose tissues harvested from the bilateral inguinal regions of Wistar rats were used as ADSCs. Rats were randomly divided into control and ADSC groups to investigate the efficacy of ADSC transplantation for wound healing (n=20 per group). Soft tissue defects were created on the heads of the rats and were covered with artificial dermis with or without the seeded ADSCs. Specimens from these rats were evaluated using digital image analysis, histology, immunohistochemistry, cell labeling, and real-time reverse-transcription polymerase chain reaction (Real-time RT-PCR).\n\nResultsThe average global wound area was significantly smaller in the ADSC group than in the control group on days 3, 7, and 14 after surgery (p<0.05). After 14 days, the blood vessel density in the wound increased by 1.6-fold in the ADSC group compared with that in the control group (p<0.01). Real-time RT-PCR results showed higher Fgfb and Vegf expression levels at all time points, and higher Tgfb1 and Tgfb3 expression levels until 14 days after surgery, in the ADSC group than in the control group (p<0.05).\n\nConclusionsIn wounds with exposed bone, autogenic ADSCs can promote vascularization and wound healing. Use of this cell source has multiple benefits, including convenient clinical application and lack of ethical concerns.

animal behavior and cognition↗

bioRxiv2019

Comparison of muscle activity, strength and balance, before and after a 6-month training using the FIFA11+ program (part 2)

PurposeSports injuries can significantly impact an athletes career, as well as impose a high financial burden on teams. Therefore, the prevention of sports injuries is an essential aspect of sports medicine. To evaluate the effects of a 6-month training period, using part 2 of the FIFA11+, on the activation and strength of core and lower limb muscles, and on static and dynamic balance performance. Materials and MethodsEight college male soccer players, 20.4{+/-}0.5 years old, completed the FIFA11+ program (part 2) at least 3x per week for 6 months. The following variables were measured, before and after the 6-month training program: activity of more than 30 muscles (with a focus on core and lower limb muscles), measured using the standardized uptake values of 18F-fluorodeoxyglucose (FDG) on positron emission tomography (PET-CT); isokinetic strength of the knee flexor and extensor and hip abductor muscles, measured at 60{degrees}/s; static balance over a 60-s period, measured using a Gravicorder; and dynamic balance, measured using the Star Excursion Balance Test. ResultsTraining improved activity levels of core (obliquus externus abdominis and erector spinae) and lower limb (tibialis anterior of the both legs) muscles (p[≤]0.03), corrected the between-limb difference in activation of the semimembranosus and improved dynamic balance, with a greater training effect on the non-dominant limb (p[≤]0.02). Training also improved knee flexor force of the non-dominant lower limb (p=0.02). ConclusionRoutine performance of the FIFA11+ (part 2) program can improve activation of core and lower limb muscles, with a concomitant improvement in dynamic balance.

physiology↗

bioRxiv2019

The synergistic antitumor effect of combined Anti-Human Epidermal Growth Factor Receptor 2 (HER2) antibody and Gamma Interferon therapy to Ab resistant breast cancer cells

Anti-HER2 antibody is molecular targeted antibody for cancer therapy. Approximately 20% of breast cancers are characterized by overexpression of HER2 protein. However, the recurrence rate was 30% and the metastasis rate was 18% one year after treatment of Anti-HER2 antibody for HER2 positive breast cancer. The resistance to antibody treatment is a major problem for patients. We previously reported that Anti-HER2 antibody and Gamma Interferon (IFN-{gamma}) combined therapy showed higher anti-tumor effect than usual therapy in vitro and in vivo mouse experiments. In this study, we evaluated whether anti-HER2 antibody and IFN-{gamma} combined therapy shows good synergistic effect against drug resistant HER2 positive breast cancer cells and higher antitumor effect than conventional clinical treatment. The resistant cell lines were made under the continuous presence of antibody until cell growth was not affected by the drug. We divided the resistant cells into the appropriate number of groups, which we and treated with anti-cancer therapy. We evaluated the antitumor effect for both in vitro study and in vivo mouse xenograft model prepared with the same immunogenicity. And we investigated the differences of immunofluorescence staining of CD8, Gr-1 and PDL-1 in tissues, especially related to immunity system. The combined therapy showed significantly higher anti-tumor effect than other groups in vitro and in vivo experiments. The combined therapy affects anti-tumor immunity in this immunofluorescence experiment. Taken together, we showed the possibility that combined therapy could be an effective treatment option for anti-HER2 antibody resistant breast cancer, helping patients suffering from cancer progression after developing treatment resistance.

cancer biology↗