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Tsouloufi, T. K.

Publications and source records attributed to Tsouloufi, T. K..

2 recordsLinked to original sources

Systematic map of the most recent evidence (2010 to 2019) on ruminant production-limiting disease prevalence and associated mortality in Ethiopia

Ethiopias livestock sector supports the livelihoods of millions of smallholder farmers. However, despite the improvements of recent years, livestock productivity remains low due to critical constraints, including infectious diseases. The aim of this study was to collate and synthesise the published evidence on ruminant disease frequency and disease-associated mortality in Ethiopia, by identifying knowledge gaps and clusters in the literature to provide the basis for a decision-making tool. Searches on both bibliographic and organisation databases were conducted in English and were restricted to the period 2010-2019. Search results were screened for relevance at title, abstract and full text level, which identified 716 articles relevant to the research question. The systematic map revealed an increased publication output from 2012-2017, compared to 2010-2011 and 2018-2019. Most studies were conducted in Oromia, Amhara and SNNPR. A substantial body of evidence was found for trypanosomosis, ectoparasite infestation, fasciolosis, nematodiasis, echinococcosis, brucellosis and bovine brucellosis. This study suggests that despite the high output of epidemiological publications, further understanding of a considerable number of diseases is required and where evidence is abundant, synthesis of information should be carried out in order to better inform decisions on disease control priorities in the livestock sector.

scientific communication and education↗

Capillary hemoglobin electrophoresis of healthy and anemic dogs: quantification, validation, and reference intervals of hemoglobin fractions

Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in hemoglobin genes research, the studies on canine hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine hemoglobin and the possible effect of age, sex, and anemia using a capillary electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for hemoglobin analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0 and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not correlated with age (P=0.866). No differences were observed in the median HbA0 and HbA2 between the two sexes (P=0.823), and healthy and anemic dogs (P=0.805). In conclusion, the capillary electrophoresis revealed a major hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, or anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

pathology↗