Biology subjects

Tran, V. N. H.

Publications and source records attributed to Tran, V. N. H..

3 recordsLinked to original sources

bioRxiv2026

Quantitative Neuropeptidomics Reveals Thermal Acclimation-Induced Remodeling of Peptidergic Signaling in the American Lobster Homarus americanus

Global warming and rising ocean temperatures pose substantial challenges to marine ecosystems and crustacean populations. As an ectothermic species, the American lobster (Homarus americanus) relies on physiological and neurochemical mechanisms to maintain homeostasis under varying environmental conditions. To elucidate the role of neuropeptides in neuronal plasticity and systemic adaptation to temperature fluctuations, we employed a quantitative mass spectrometry-based approach to probe key neuropeptides involving thermal adaptation in four lobster neural tissues at three temperatures: 4 {degrees}C (cold), 11 {degrees}C (control), and 18 {degrees}C (warm). Peptidomic profiling revealed a global reduction in peptide abundance during cold exposure, alongside coordinated, tissue-specific reconfigurations of the neuropeptidome between experimental groups. Cold exposure led to a significant downregulation of RFamide, leucokinin, and pyrokinin peptides in the commissural ganglia, whereas B-type allatostatin (AST-B), natalisin, and RYamide peptides were drastically elevated in the brain of warm-acclimated animals, with comparatively fewer detectable peptide abundance changes in the sinus gland and the stomatogastric ganglion. Collectively, our findings elucidate neuropeptide signaling pathways underlying thermal tolerance and adaptive resilience in Homarus americanus, offering insights into the survival mechanism and neurochemical basis of neural circuits in response to thermal acclimation. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=108 SRC="FIGDIR/small/710231v1_ufig1.gif" ALT="Figure 1"> View larger version (24K): org.highwire.dtl.DTLVardef@a3d24borg.highwire.dtl.DTLVardef@1434aaaorg.highwire.dtl.DTLVardef@db11cforg.highwire.dtl.DTLVardef@6e5995_HPS_FORMAT_FIGEXP M_FIG C_FIG

neuroscience↗

bioRxiv2025

cNPDB: A comprehensive empirical crustacean neuropeptide database

Neuropeptides, key signaling molecules essential for dynamic regulation of biological processes, have been studied via crustacean model systems for more than 40 years. We present cNPDB, the first centralized resource dedicated to this dynamic area of research, promisingly accelerating crustacean neuropeptidome research and offering a blueprint for cross-species translational studies of neuropeptide signaling. cNPDB is comprised of 1364 published neuropeptides from 29 crustacean species, each annotated with corresponding taxonomic information, computed physicochemical properties, spatial localization, and predicted three-dimensional structure. The intuitive web-interface supports keyword and property-based filtering, sequence alignment, property calculation, and links to available mass spectrometry imaging data, streamlining discovery. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=109 SRC="FIGDIR/small/667494v1_ufig1.gif" ALT="Figure 1"> View larger version (40K): org.highwire.dtl.DTLVardef@ec5445org.highwire.dtl.DTLVardef@19084a3org.highwire.dtl.DTLVardef@f4228dorg.highwire.dtl.DTLVardef@89504_HPS_FORMAT_FIGEXP M_FIG C_FIG

bioinformatics↗

bioRxiv2025

HILIC-Enabled Mass Spectrometric Discovery of Novel Endogenous and Glycosylated Neuropeptides in the American Lobster Nervous System

Neuropeptides are a highly conserved and diverse class of intercellular signaling molecules that regulate a broad range of neural and hormonal processes across animal phyla. The American lobster, Homarus americanus, has long served as a powerful invertebrate model for the discovery and functional investigation of neuropeptides. Among common post-translational modifications (PTMs) found in neuropeptides, glycosylation remains underexplored due to the inherently low in vivo abundance and intrinsically complex structural heterogeneity. In this study, we employed hydrophilic interaction liquid chromatography (HILIC) enrichment coupled with oxonium-ion triggered EThcD fragmentation strategy to simultaneously profile novel endogenous and glycosylated neuropeptides across eight distinct neural tissues and neuroendocrine organs of Homarus americanus. This integrative mass spectrometry-based approach led to the identification of 154 endogenous neuropeptides derived from 25 families, approximately one-third of which are newly reported, and uncovered 24 glycosylated neuropeptides in this species for the first time. These peptides exhibit strong tissue-specific expression, distinct proteolytic cleavage patterns, and confidently localized glycosylation sites. Our results highlight the utility of integrated sampling enrichment and hybrid fragmentation strategies for deep neuropeptidomic profiling and provide a valuable resource for future studies on the functional roles of newly identified neuropeptides and glycosylation in crustacean neuromodulation and peptidergic signaling. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=108 SRC="FIGDIR/small/661634v2_ufig1.gif" ALT="Figure 1"> View larger version (20K): org.highwire.dtl.DTLVardef@9a1745org.highwire.dtl.DTLVardef@6decc4org.highwire.dtl.DTLVardef@d34256org.highwire.dtl.DTLVardef@1a83b4c_HPS_FORMAT_FIGEXP M_FIG C_FIG

neuroscience↗