Biology subjects

Thakur, R. K.

Publications and source records attributed to Thakur, R. K..

4 recordsLinked to original sources

bioRxiv2020

Experiences of cervical cancer survivors in Chitwan, Nepal: a qualitative study

Introduction Cervical cancer is a global leading cause of morbidity and mortality. The majority of cervical cancer deaths occur in developing countries including Nepal. Though knowledge of cervical cancer is an important determinant of women’s participation in prevention and screening for cervical cancer, little is known about this topic in Nepal. This study explores the experiences of cervical cancer survivors and assesses the attitude of family and community towards it and stigma related to this disease in Bharatpur, Nepal.Methods The study design is qualitative methods involving two focus-group discussions. A total of 17 cervical cancer survivors were selected purposively from Chitwan. All qualitative data were transcribed and translated into English and were thematically analyzed.Results Majority of the participants had scant knowledge about cervical cancer, its causative agent, showed less cervical cancer screening, delayed healthcare-seeking behavior despite having persistent symptoms before the diagnosis. The main reasons identified for not uptaking the cervical screening methods are an embarrassment and having no symptoms at all. Most of them endured social stigma related to cervical cancer in the form of physical isolation and verbal abuse.Conclusions There is an urgent need for interventions to make women and the public aware of cervical cancer and launch effective health education campaigns, policies for cervical cancer prevention programs. This implementation can save the lives of hundreds of women and help them avoid going through all the negative experiences related to cervical cancer. More studies are required to gain the perspectives, knowledge, experiences, and attitudes of cervical cancer survivors to add to the research.View Full Text

scientific communication and education↗

bioRxiv2020

Concurrent stem- and lineage-affiliated chromatin programs precede hematopoietic lineage restriction

The emerging notion of hematopoietic stem- and progenitor cells (HSPCs) as a low-primed cloud without sharply demarcated gene expression programs raises the question on how cellular fate options emerge, and at which stem-like stage lineage priming is initiated. Here we investigated single-cell chromatin accessibility of Lineage-, cKit+, Sca1+ (LSK) HSPCs spanning the early differentiation landscape. Application of a signal-processing algorithm to detect transition points corresponding to massive alterations in accessibility of 571 transcription factor-motifs revealed a population of LSK FMS-like tyrosine kinase 3(Flt3)intCD9high cells that concurrently display stem-like and lineage-affiliated chromatin signatures pointing to a simultaneous gain of both Lympho-Myeloid and Megakaryocyte-Erythroid programs. Molecularly and functionally, these cells position between stem cells and committed progenitors, display multi-lineage capacity in vitro and in vivo, but lack self-renewal activity. This integrative molecular analysis resolves the heterogeneity of cells along hematopoietic differentiation and permits investigation of chromatin-mediated transition between multipotency and lineage restriction.

cell biology↗

bioRxiv2018

Studies on effect of gold nanoparticles on Meloidogyne incognita and tomato plants growth and development

The plant parasitic nematodes are one of world major agricultural pest, causing in excess of 157 billion dollars in worldwide damage annually. This study has provided evidence that gold nanoparticles have great utility for management of root-knot nematodes in tomato crop. The effect of gold nanoparticles on Meloidogyne incognita J2 was remarkable under the direct exposure in water, after three hours of incubation of Meloidogyne incognita with GNPs showed the 100% mortality. The lesser survival rate of Meloidogyne incognita in soil treatment showed the strong nematicidal effect of gold nanoparticles. Subsequently, the pot experiment had shown the beneficial effects of gold nanoparticles for intensively managing the root-knot nematode. The Pot experiment not only showed us that GNPs were lethal to root-knot nematodes were also induces growth of tomato plants and didnt have any kind of negative impact on plant growth. In our study, GNPs were found to be safe and lethal to Meloidogyne incognita.

plant biology↗

bioRxiv2017

Exploration of Western Himalayan region for identification of gold nanoparticles synthesizing bacteria

The development of eco-friendly methods for the synthesis of nanomaterial shape and size is an important area of research in the field of nanotechnology. In present study an indigenous bacterial strain GPI-1 has been isolated from a local gold mine Khaltunala. It was characterized morphological, biochemically and also by using 16S rrna gene technology and was identified as Bacillus flexus GPI-1, the phylogeny of this bacterial strain was determined using various bioinformatics tools viz BLASTn and MEGA 5.0. To achieve maximum invitro gold nanoparticles synthesis various parameters such as pH, incubation temperature, incubation time period and wavelength were optimized as 6.8, 37{degrees}C, 36 hrs, 560 nm respectively. Stable and cubical gold nanoparticles (GNPs) formation with 40-45 nm dimensions were synthesized successfully under invitro conditions upon exposure of gold chloride trihydrates (HAuCl4) solution to the supernatant of Bacillus flexus strain GPI-1. These gold nanoparticles have been characterized by Transmission electron microscope, Fourier transform infrared spectroscopy (FTIR). Thus in the present study successful biosynthesis method of stable and cubical gold nanoparticles in the size range of 12-30 nm using this efficient Bacillus flexus GPI-1 strain. Thus in the present study leading to development of an easy bioprocess for synthesis of GNPs of desired size and shape, has been reported and this green route of biosynthesis of GNPs is a simple, economically viable and an eco-friendly process. The use of gold nanoparticles in biomedical research like X-ray computed tomography and magnetic resonance imaging, cancer research, drug delivery applications.

microbiology↗