RNA-dependent RNA amplification as a strategy to perform tRNA sequencing on low-input samples
Transfer RNAs (tRNAs) are key molecules that deliver amino acids to the translating ribosome according to their cognate codon encoded in messenger RNA (mRNA). Due to the modified nature of tRNA nucleotides, accurate tRNA quantification can be tedious, especially when dealing with small sample inputs. Here, we took advantage of an RNA-dependent RNA amplification method using T7 polymerase to quantify tRNA abundance in low biological input. Our method, called T7 High-resolution original RNA (Thor)-tRNA-Seq, showed reproducible and quantitative measurement of low tRNA inputs. Thus, our Thor-tRNA-Seq is a robust and reliable approach for the quantification of tRNA in samples with small and precious biological material.
molecular biology↗