Biology subjects

Tako Djimefo, A. K.

Publications and source records attributed to Tako Djimefo, A. K..

4 recordsLinked to original sources

bioRxiv2026

Green-synthesized silver nanoparticles enhance Guibourtia tessmannii antithromboinflammatory therapeutic potential

IntroductionThromboinflammation, which represents the pathological interplay between inflammation and thrombosis, is a leading cause of global mortality. Current therapies are frequently associated with an increased risk of bleeding and do not adequately address the inflammatory component of the disease. The African tree Guibourtia tessmannii represents a promising source of natural anti-inflammatory compounds. This study aimed to synthesize and characterize silver nanoparticles using an aqueous bark extract of G. tessmannii (GT-AgNPs) and to evaluate their anti-inflammatory and anticoagulant properties. MethodsGT-AgNPs were synthesized by reducing silver nitrate with an aqueous extract of G. tessmannii bark. The nanoparticles were comprehensively characterized using UV-Vis spectroscopy, FTIR spectroscopy, powder X-ray diffraction, and scanning electron microscopy. In vitro anti-inflammatory activity was evaluated through inhibition of bovine serum albumin denaturation, whereas in vivo anti-inflammatory activity was assessed using the carrageenan-induced rat paw edema model. Anticoagulant activity was investigated by measuring activated partial thromboplastin time (aPTT) and prothrombin time (PT), corresponding to the intrinsic and extrinsic coagulation pathways, respectively. ResultsThe synthesis successfully produced GT-AgNPs with an average particle size of approximately 20 nm. Both the aqueous extract and GT-AgNPs exhibited marked anti-inflammatory activity. The nanoparticles achieved 95% inhibition of protein denaturation in vitro and 95% inhibition of carrageenan-induced paw edema in vivo at a dose of 0.4 mg/kg body weight after 5 h. Furthermore, both the extract and GT-AgNPs demonstrated dose-dependent anticoagulant activity. ConclusionThe study demonstrated that GT-AgNPs, synthesized from the bark of G. tessmannii, possess significant anti-inflammatory and anticoagulant properties. These findings highlight the potential of GT-AgNPs as nanotherapeutic candidates for the management of thrombo-inflammatory disorders.

pharmacology and toxicology↗

bioRxiv2026

Mimosa pudica-derived zinc oxide nanoparticles preserve mesenchymal stromal cell viability, morphology, and osteogenic competence

IntroductionMusculoskeletal disorders remain a major cause of disability worldwide and require non invasive regenerative strategies that support tissue repair. Green-synthesized zinc oxide nanoparticles (ZnONPs) have attracted interest because of their biocompatibility and biological activity. This study investigated the synthesis of Mimosa pudica-derived ZnONPs (ZnOMP) and evaluated their effects on human bone marrow mesenchymal stromal cells (BM-MSCs). MethodologyZnOMP were synthesized using an aqueous extract of Mimosa pudica leaves and characterized by UV-Vis spectroscopy, FTIR spectroscopy, powder X-ray diffraction, SEM, EDS, and TEM. BM-MSCs isolated from human bone marrow were exposed to ZnOMP, plant extract, and synthesized ZnO nanoparticles. Cell metabolic activity was assessed by MTT assay after 1, 3, and 5 days. Cytoskeletal and nuclear morphology were analyzed by fluorescence microscopy and CellProfiler-based morphometry. Osteogenic differentiation was evaluated after 21 days using Alizarin Red S staining and quantification. ResultsSpectroscopic and microscopic analyses confirmed the successful formation of phytochemical-capped ZnOMP nanoparticles with nanoscale dimensions and specific elemental composition. ZnOMP maintained significantly higher metabolic activity than Mimosa pudica extract or ZnO at both 150 and 300 g/mL. Morphometric profiling revealed that Mimosa pudica extract induced the most pronounced changes in nuclear morphology, reflecting enhanced nuclear plasticity and substantial remodeling of nuclear architecture, whereas ZnOMP preserved cellular and nuclear features closer to untreated controls. During osteogenic induction, ZnOMP did not impair matrix mineralization and preserved the ability of BM-MSCs to form a mineralized extracellular matrix. ConclusionMimosa pudica-mediated ZnO nanoparticles combine favorable biocompatibility with preservation of mesenchymal stem cell morphology and osteogenic competence. These findings support their potential use as bioactive nanomaterials for musculoskeletal tissue engineering and regenerative medicine.

pharmacology and toxicology↗

bioRxiv2024

Cylicodiscus gabunensis (FABACEAE) aqueous stem bark extract mediated silver nanoparticle enhance anti-inflammation on Wistar rats

IntroductionPlants are a source of bioactive ingredient that can play a key role in development of new drugs. In recent years, plant mediated-biological synthesis of nanoparticles has gained importance due to its simplicity, cost effectiveness and eco-friendly nature. To the best of our readings, nanoparticule from Cylicodiscus gabunensis stem bark is still untaped. This work therefore aimed at assessing the anti-inflammatory properties of the silver nanoparticles obtained from the aqueous extract of the stem bark of Cylicodiscus gabunensis(Cg). MethodologyCylicodiscus gabunensis extract was prepared by infusion-followed by the biosynthesis of silver nanoparticles. The synthesis was monitored by color and UV-Vis spectrophotometry. Infrared spectroscopy aimed at revealing the functional groups present at the surface of the nanoparticles. Structural elucidation was done by powder X-ray crystallography, while microstructure and elemental mapping was performed with scanning electron microscopy and energy dispersive X-ray spectroscopy. In vitro anti-inflammatory test was done using the BSA denaturation based test. The acute toxicity was done using the OEDC 425 guideline. carrageenan-induced rat paw oedema model was used to ascertain the in vivo anti-inflammatory effects. ResultsPhytochemical screening of the aqueous extract of Cylicodiscus gabunensis revealed the presence of polyphenols, flavonoids, alkaloids, coumarins, saponins, triterpenes, steroids, reducing sugar, tannins, and the absence of anthraquinone. The surface Plasmon resonance peak in the UV-Vis spectrum shows absorption spectra between 380 and 550 nm. Stability studies done over time showed that the nanoparticles were stable even after two months of synthesis. IR spectroscopy revealed the presence of O-H, N-H, C{equiv}C, C=C, C-O, and C=O groups. PXRD confirms the formation of silvernanoparticles (AgNPs) while nanograins of various forms were visualized by SEM and EDX. No toxic sign was observed. The maximum inhibitory percentages were 95% at 200 {micro}g/mL and 91% at 400 g/Kg for in vitro and in vivo anti-inflammatory effects, respectively. ConclusionThis paper spot the light on silver nanoparticle from Cylicodiscus gabunensis aqueous stem bark for their anti-inflammatory effect on paw oedema model.

pharmacology and toxicology↗

bioRxiv2024

Anti-inflammatory assessment of zinc oxide nanoparticles mediated Aframomum citratum (C. Pereira) K. Schum (Zingiberaceae) in Wistar rats

IntroductionZinc oxide nanoparticles (ZnONPs) have been synthesized using a wide range of techniques, including green chemistry, because of their versatility, cost effectiveness, and environmentally friendly nature, offering thereby interesting and inexpensive therapeutic options. This study aimed to develop zinc oxide nanoparticles as an anti-inflammatory agent using Aframomum citratum seed extract. MethodologyZnONPs were prepared by the reaction between zinc nitrate and an alkalineaqueous extract of A. citratum seeds. The isolated nanoparticles were then characterized using UV-Vis, FTIR, SEM/EDX, PXRD and TEM techniques. The toxicological profile was assessed at a limited dose of 2000 mg/kg in rats, and methods for heat denaturation of egg albumin, stabilization of red blood cell membranes and inhibition of carrageenan-induced plantar oedema were studied to assess anti-inflammatory properties. ResultsThe formation of ZnONPs was observed by a color change and the appearance of the plasmon resonance peak at 360 nm in the UV-Vis spectrum while FTIR confirmed the presence of secondary metabolites; SEM confirmed the presence of multiform aggregates, and TEM visualize point like particles. EDS confirmed the presence of Zn atoms within the synthetized material. The toxicological profile studied showed no harmful signs; zinc oxide nanoparticles synthesized from A. citratum seed extract showed high inhibition percentages of 86 (1mg/mL); 77 (0.6mg/mL) and 79(1mg/mL) when subjected to inhibition of heat-induced egg albumin denaturation, red cell membrane stabilization and oedema induction by carrageenan respectively, not significatively different compared with diclofenac sodium as positive controls. ConclusionZinc oxide nanoparticles synthesized and characterized from A. citratum seed extract act as a potent anti-inflammatory agent and are devoid of acute oral toxicity.

pharmacology and toxicology↗