Biology subjects

Takeda, A. K.

Publications and source records attributed to Takeda, A. K..

3 recordsLinked to original sources

bioRxiv2023

Temperament in early childhood is associated with gut microbiota composition and diversity

Temperament is a key predictor of human mental health, and cognitive and emotional development. While human fear behavior is reportedly associated with gut microbiome in infancy, infant gut microbiota changes dramatically during the first five years, when the diversity and composition of gut microbiome are established. This period is crucial for developing human executive functioning, including emotion regulation. Therefore, this study investigated the relationship between temperament and gut microbiota in 284 preschool children aged 3-4 years. Child temperament was assessed by maternal reports of the Childrens Behavior Questionnaire. Gut microbiota (alpha/beta diversity and genera abundance) was evaluated using 16S rRNA sequencing of stool samples. A high abundance of anti-inflammatory bacteria (e.g., Faecalibacterium) and low abundance of inflammatory bacteria (e.g., Eggerthella, Flavonifractor) were associated with higher positive emotionality and reward-seeking (i.e., Surgency/Extraversion, {beta} =0.15, p = 0.013), and lower negative emotionality and behavioral inhibition (i.e., Negative Affectivity, {beta} = -0.17, p = 0.004). Additionally, gut microbiota diversity was associated with a more active approach and exploration (i.e., Impulsivity, a specific aspect of Surgency/Extraversion, {beta} = 0.16, p = 0.008). This study provides insight into the biological mechanisms of temperament and takes important steps toward identifying predictive markers of psychological/emotional risk.

microbiology↗

bioRxiv2022

The Intestinal Microbiome, Dietary Habits, and Physical and Psychological Resilience in Postpartum Women

The population of postpartum women suffering from mental illness is increasing steadily, particularly under conditions of the COVID-19 pandemic. Identifying factors that contribute to resilience in postpartum women is urgently needed to decrease risks of poor physical and psychological functioning. Studies have linked variations in the intestinal microbiota to depression in clinical samples, but the impacts in postpartum women in a Japanese population are unknown. We conducted two studies to examine the links between intestinal microbiota, physical condition, and psychological state in nonclinical, postpartum Japanese women. Our results show that decreasing Lachnospira and alpha diversity of microbiome is related to high mental health risk (i.e., parenting stress and/or depression). Psychological resilience and physical conditions were associated with relative abundances of genera Blautia, Clostridium, Eggerthella. This study contributes to further understanding of the gut-brain axis mechanisms and supports proposals for interventions to enhance resilience in postpartum women.

microbiology↗

bioRxiv2020

Analysis of healthy reference ranges for clinically relevant gut microbial indicators: A large web-based cohort study in Japan

BackgroundAlthough several large cohort studies have focused on the human gut microbiome, large cohort study of the Japanese gut microbiome is scarce, especially that of healthy or non-diseased individuals. The purpose of this study was to establish a reference range for gut microbial indices by collecting Japanese real-world microbiome data from a Mykinso cohort. MethodsWe collected stool samples and original survey on lifestyle from 5,843 Japanese people through Mykinso gut microbiome testing service. From the obtained 16S rRNA sequence data derived from the stool sample, the ratio and distribution of each taxon were analyzed. The relationship between different epidemiological attributes and gut microbial indicators were statistically tested. ResultsThe qualitative and quantitative indicators of these common gut microbiota were confirmed to be strongly correlated with age, sex, constipation/diarrhea, and history of lifestyle-related diseases. Therefore, we set up the healthy sub-cohort that controlled for these attribute factors, and defined a reference range from the distribution of gut microbial index in that population. ConclusionsThe gut microbiota of Japanese people had high beta diversity, and there was no single "typical" gut microbiota type. We believe that the reference range of the gut microbial index obtained in this study can be a new reference value for determining the balance and health of the gut microbiota of an individual. In the future, it is necessary to clarify the clinical validity of this reference value by comparing with the clinical disease cohort.

microbiology↗