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Summer, R.

Publications and source records attributed to Summer, R..

3 recordsLinked to original sources

Latexin facilitates neointimal formation via promoting smooth muscle cell proliferation and macrophage migration

RationaleNeointimal formation is the major cause of cardiovascular diseases such as atherosclerosis and restenosis. Although restenosis rates are significantly reduced by the development of drug-eluting, the stent may not be implanted at all atherosclerotic sites, and long-term dual anti-platelet therapy is needed after stent implantation. Thus, it is essential to elucidate the detailed mechanisms underlying neointimal formation for developing novel therapies. Latexin (LXN), a previously identified pro-inflammatory protein, is highly expressed in vasculature and its expression is regulated by shear stress. Whether it plays a role in vascular remodeling, however, remains unknown. ObjectiveTo determine whether LXN is involved in neointimal formation, and if so, to define the molecular mechanisms involved. Methods and ResultsWe found that the expression of LXN was significantly increased in neointimal hyperplasia, as determined by western blot. Immunofluorescent staining indicated that increased LXN expression was predominantly localized in smooth muscle cells and macrophages. To determine the cell specific roles of LXN in neointimal formation after injury, we generated global, smooth muscle cell (SMC)-specific, endothelial specific-, and myeloid-specific LXN knockout (KO) mice. We found that global, SMC-, and myeloid-specific LXN deficiency markedly prevented neointimal hyperplasia in mice after carotid artery ligation, whereas LXN deficiency in endothelial cells had no effects. Mechanistically, we found that LXN deficiency in SMCs significantly attenuated SMC proliferation and migration, mainly through inhibiting the expression of platelet-derived growth factor (PDGF) receptors. Intriguely, LXN deficiency in macrophages inhibited monocyte chemoattractant protein-1 (MCP-1)-induced macrophage migration through inhibiting ERK phosphorylation. ConclusionsIn summary, we for the first time demonstrated that LXN is essentially involved in SMC proliferation and microphage migration. Specific inhibition of LXN signaling may provide a novel therapeutic strategy for the treatment of cardiovascular diseases, such as restenosis and atherosclerosis. Novelty and SignificanceO_ST_ABSWhat is known?C_ST_ABSO_LILatexin (LXN) is considered to be a novel pro-inflammatory protein and its expression is responsive to laminar shear stress. C_LIO_LIVascular inflammation, SMC proliferation, and macrophage migration are critical events in neointimal hyperplasia. C_LI What new information does this article contribute?O_LILXN is elevated in carotid artery ligation-induced neointimal hyperplasia in mice. C_LIO_LISMC-specific LXN deficiency prevents neointimal hyperplasia through inhibiting SMC proliferation migration via attenuating platelet-derived growth factor receptor expression. C_LIO_LIMyeloid-specific LXN deficiency prevents neointimal hyperplasia through inhibiting monocyte chemoattractant protein-1-induced macrophage migration and extracellular signal-regulated kinase phosphorylation. C_LI Neointimal formation is the major cause of cardiovascular disease such as atherosclerosis and restenosis after stenting or balloon angioplasty. Because of the development of drug-eluting stents, restenosis rates have been significantly reduced; however, it is still essential to explore the detailed mechanisms underlying neointimal hyperplasia. Latexin (LXN) is considered a novel pro-inflammatory protein that is ubiquitously expressed in vascular and immune cells. We found that LXN is elevated in neointimal hyperplasia, and its deficiency in SMCs and macrophages, but not in ECs, markedly prevents neointimal formation after carotid artery ligation in mice. Mechanistically, LXN deficiency in SMCs prevents SMC proliferation and migration via attenuation of platelet-derived growth factor receptor expression. Further, myeloid-specific LXN deficiency inhibits monocyte chemoattractant protein-1-induced macrophage migration via attenuation of extracellular signal-regulated kinase phosphorylation. For the first time, the present study demonstrated that LXN is a crucial mediator implicated in SMC biology and macrophage migration.

physiology↗

Inhibiting endothelial cell Mst1 attenuates acute lung injury in mice

BackgroundLung endothelium plays a pivotal role in the orchestration of inflammatory and injury responses to acute pulmonary insults. Mammalian sterile 20-like kinase 1 (Mst1), a mammalian homolog of Hippo, is a serine/threonine kinase that is ubiquitously expressed in many tissues and has been shown to play an important role in the regulation of apoptosis, inflammation, stress responses, and organ growth. While Mst1 exhibits high expression in the lung, its involvement in the endothelial response to pulmonary insults remains largely unexplored. MethodsMst1 activity was assessed in lung endothelium by western blot. Mst1 endothelial specific knockout mice and a pharmacological inhibitor were employed to assess the effects of Mst1 on homeostatic and lipopolysaccharide (LPS)-induced endothelial responses. Readouts for these studies included various assays, including NF-{kappa}B activation and levels of various inflammatory cytokines and adhesion molecules. The role of Mst1 in lung injury was evaluated in a LPS-induced murine model of acute lung injury (ALI). ResultsMst1 phosphorylation was significantly increased in lung endothelial cells after exposure to tumor necrosis factor (TNF)-alpha (TNF-) and mouse lung tissues after LPS exposure. Overexpression of full length Mst1 or its kinase domain promoted nuclear factor kappaB (NF-{kappa}B) activation through promoting JNK and p38 activation, whereas dominant negative forms of Mst1 (DN-Mst1) attenuated endothelial responses to TNF- and interleukin-1{beta}. Consistent with this, targeted deletion of Mst1 in lung endothelium reduced lung injury to LPS in mice. Similarly, wild-type mice were protected from LPS-induced lung injury following treatment with a pharmacological inhibitor of Mst1/2. ConclusionsOur findings identified Mst1 kinase as a key regulator in the control of lung EC activation and suggest that therapeutic strategies aimed at inhibiting Mst1 activation might be effective in the prevention and treatment of lung injury to inflammatory insults.

physiology↗

Proteomic Profile of TGF-β1 treated Lung Fibroblasts identifies Novel Markers of Activated Fibroblasts in the Silica Exposed Rat Lung

We performed liquid chromatography-tandem mass spectrometry (LC-MS/MS) on control and TGF-{beta}1-exposed rat lung fibroblasts to identify proteins differentially expressed between cell populations. A total of 1648 proteins were found to be differentially expressed in response to TGF-{beta}1 treatment and 196 proteins were expressed at [≥] 1.2 fold relative to control. Guided by these results, we next determined whether similar changes in protein expression were detectable in the rat lung after chronic exposure to silica dust. Of the five proteins selected for further analysis, we found that levels of all proteins were markedly increased in the silica-exposed rat lung, including the proteins for the very low density lipoprotein receptor (VLDLR) and the transmembrane (type I) heparin sulfate proteoglycan called syndecan 2 (SDC2). Because VLDLR and SDC2 have not, to our knowledge, been previously linked to the pathobiology of silicosis, we next examined whether knockdown of either gene altered responses to TGF-{beta}1 in MRC-5 lung fibroblasts. Interestingly, we found knockdown of either VLDLR or SDC2 dramatically reduced collagen production to TGF-{beta}1, suggesting that both proteins might play a novel role in myofibroblast biology and pathogenesis of silica-induced pulmonary fibrosis. In summary, our findings suggest that performing LC-MS/MS on TGF-{beta}1 stimulated lung fibroblasts can uncover novel molecular targets of activated myofibroblasts in silica-exposed lung.\n\nHighlightsWe identified 196 proteins differentially expressed between control and TGF-{beta}1 treated fibroblastsby LC-MS/MS.\n\nSeveral proteins identified by LC-MS/MS were also found to be differentially expressed in whole lung tissues and isolated fibroblasts after chronic exposure to silica dust, including the very low density lipoprotein receptor (VLDLR) and the transmembrane type I heparan sulfate proteoglycan called syndecan 2\n\nKnockdown of SDC2 or VLDLR markedly inhibited collagen production in MRC-5 fibroblasts, suggesting a novel pathogenic role for these proteins in myofibroblast biology.

pharmacology and toxicology↗