Biology subjects

Soukup, A.

Publications and source records attributed to Soukup, A..

3 recordsLinked to original sources

bioRxiv2020

The Arabidopsis TTL3 protein interconnects brassinosteroid signalling and cytoskeleton during lateral root development

Lateral roots are essential components of the plant edaphic interface, contributing to water and nutrient uptake, biotic and abiotic interactions, stress survival, and plant anchorage. We have identified the TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE 3 (TTL3) being related to lateral root emergence and later development. TTL3 interacts with microtubules and potentially interconnects cytoskeletal function with the brassinosteroid signalling pathway. Loss of function of TTL3 leads to a reduced number of emerged lateral roots due to delayed development of lateral root primordia. Lateral root growth of the ttl3 mutant is less sensitive to BR treatment. Timing and spatial distribution of TTL3 expression is consistent with its role in development of lateral root primordia before their emergence and subsequent development into lateral roots. TTL3 is a novel component of the root system morphogenesis regulatory network.

developmental biology↗

bioRxiv2017

A LaeA- and BrlA-dependent cellular network governs tissue-specific secondary metabolism in the human pathogen Aspergillus fumigatus

Biosynthesis of many ecologically important secondary metabolites (SMs) in filamentous fungi is controlled by several global transcriptional regulators, like the chromatin modifier LaeA, and tied to both development and vegetative growth. In Aspergillus molds, asexual development is regulated by the BrlA>AbaA>WetA transcriptional cascade. To elucidate BrlA pathway involvement in SM regulation, we examined the transcriptional and metabolic profiles of {Delta}brlA, {Delta}abaA, {Delta}wetA and wild-type strains of the human pathogen Aspergillus fumigatus. We find that BrlA, in addition to regulating production of developmental SMs, regulates vegetative SMs and the SrbA-regulated hypoxia stress response in a concordant fashion to LaeA. We further show that the transcriptional and metabolic equivalence of {Delta}brlA and {Delta}laeA is mediated by a LaeA requirement preventing heterochromatic marks in the brlA promoter. These results provide a framework for the cellular network regulating not only fungal SMs but diverse cellular processes linked to virulence of this pathogen.

microbiology↗

bioRxiv2017

Green microalga Trebouxia sp. produces strigolactone-related compounds

Different algal species that may have germination inducing activity of holoparasitic broomrape weeds Phelipanche aegyptiaca and P. ramosa seeds were screened through germination bioassay. Green alga produce SL-related compounds. Applied extracts of biomass obtained from the culture of green alga Trebouxia arboricola increased seeds germination of both parasites. An optimatization of the alga extraction led to an increase of P. aegyptiaca germination. Exhausted medium also contained SL-related compounds. The crude extract stimulated the roots length of Arabidopsis thaliana tested in vitro. A similar effect had the algae and GR24 aplications on expression levels of the SL-related genes in Physcomitrella patens. The novel analytical method DESI-MSI detected production of carlactone in the algae. The Trebouxia sp. culture applications in pot experiments had positive effect on growth characteristics of pea plants.

plant biology↗