Biology subjects

Sniezko, R. A.

Publications and source records attributed to Sniezko, R. A..

3 recordsLinked to original sources

bioRxiv2026

The impact of Cronartium ribicola inoculum density on quantitative disease resistance in whitebark pine.

Whitebark pine (Pinus albicaulis), a wide-ranging high-elevation conifer in western North America, is listed as threatened in the U.S. and as endangered in Canada. A major threat to whitebark pine is the non-native, invasive white pine blister rust disease, caused by the fungal pathogen Cronartium ribicola. In many pathosystems (including white pine blister rust), seedling inoculation trials are used to identify parent trees with genetic resistance. However, many of these trials use only one spore density for inoculation, and little information exists on the effectiveness of quantitative disease resistance (QDR) under varying spore densities and the corresponding implications for field performance. In this study, we examine the levels of infection and survival present within six whitebark pine seedling families previously rated for QDR (three susceptible and three resistant families) under six widely varying inoculum densities. The susceptible families showed very high infection and mortality at all inoculum densities, while performance of the resistant families varied with spore density treatment. The information gathered from the study will be useful in updating the projections of the future of whitebark pine populations under field conditions in areas of different rust hazard. The results also serve as a caution to those working in other pathosystems where seedling inoculation trials based on one spore density level are used to rate the resistance level of parent trees and their associated progeny.

genetics↗

bioRxiv2024

Genomics-driven monitoring of Fraxinus latifolia (Oregon Ash) for conservation and EAB-resistance breeding

Understanding the evolutionary processes underlying range-wide genomic variation is critical to designing effective conservation and restoration strategies. Evaluating the influence of connectivity, demographic change, and environmental adaptation for threatened species can be invaluable to proactive conservation of evolutionary potential. In this study, we assessed genomic variation across the range of Fraxinus latifolia, a foundational riparian tree native to western North America recently exposed to the invasive emerald ash borer (Agrilus planipennis; EAB). Over 1,000 individuals from 61 populations were sequenced using reduced representation (ddRAD-seq) across the species range. Strong population structure was evident along a latitudinal gradient, with population connectivity largely maintained along central valley river systems, and a center of diversity coinciding with major river systems central to the species range. Despite evidence of connectivity, estimates of nucleotide diversity and effective population size were low across all populations, suggesting the patchy distribution of F. latifolia populations may impact its long-term evolutionary potential. Range-wide estimates of genomic offset, which indicate genomic change required to adjust to future climate projections, were greatest in the eastern and lowest in the southern portions of the species range, suggesting the regional distribution of genomic variation may impact evolutionary potential longer-term. To preserve evolutionary capacity across populations needed for development of climate-resilient, EAB-resistant breeding programs, prioritizing conservation of range-wide genomic diversity will provide a foundation for species management long-term.

genomics↗

bioRxiv2017

The role of hybridization during ecological divergence of southwestern white pine (Pinus strobiformis) and limber pine (P. flexilis)

Interactions between extrinsic factors, such as disruptive selection, and intrinsic factors, such as genetic incompatibilities among loci, can contribute to the maintenance of species boundaries. The relative roles of these factors in the establishment of reproductive isolation can be examined using species pairs characterized by gene flow throughout their divergence history. We investigated the process of speciation and the maintenance of species boundaries between Pinus strobiformis and P.flexilis. Utilizing ecological niche modeling, demographic modeling, and genomic cline analyses, we illustrated a history of divergence with continuous gene flow between these species. We found an abundance of advanced generation hybrids and a lack of loci exhibiting large allele frequency differences across the hybrid zone. Additionally, we found evidence for climate-associated variation in the hybrid index and niche divergence between parental species and the hybrid zone. Our results are consistent with extrinsic factors, such as climate, being an important isolating mechanism for these species. A buildup of intrinsic incompatibilities and of co-adapted gene complexes is also apparent in our results, although these appear to be in the earliest stages of development. This supports previous work in coniferous species demonstrating the importance of extrinsic factors in creating and enforcing species boundaries. Overall, we lend support to the hypothesis that varying strengths and directions of selection pressures across the long lifespans of conifers, in combination with their life history strategies, delay the evolution of strong intrinsic incompatibilities.

evolutionary biology↗