Biology subjects

Sistla, S.

Publications and source records attributed to Sistla, S..

2 recordsLinked to original sources

bioRxiv2020

Structure of Macrolide Efflux Protein (MefA/E) in Streptococcus pneumoniae: An in silico approach

BackgroundMacrolides are one of the commonest antibiotics used to treat bacterial respiratory tract infections. Resistance to this class of antibiotics is on the rise and is mediated by macrolide efflux (MefA/E) protein as one of the mechanisms. Despite its importance, the structure of this protein is not known yet. MethodsThe publicly available MefA/E protein sequence was used to model the structure. Modelling was performed in I-TASSER, and the model was further refined. Its orientation in a membrane was studied using OPM server. Results and conclusionsThe structure of MefA/E resembled that of Major Facilitator Superfamily (MFS) proteins, with 13 transmembrane helices. It had a V-shaped conformation, with the wider part towards the outer membrane layer.

bioinformatics↗

bioRxiv2020

Genome analysis of clinical pneumococcal isolates to decipher sequence types, antibiotic resistance mechanisms and virulence gene profiles

IntroductionStreptococcus pneumoniae is a common cause of meningitis and pneumonia. Various studies have explored the relationship between genome and phenotypic characteristics like antibiotic resistance. MethodsFour pneumococcal isolates from clinical specimens were included. DNA was extracted and genomes were sequenced in 2x101bp layout. Standard genome analysis tools and pipelines were employed to understand phenotypic characteristics. ResultsThere was almost perfect agreement of genome-based predictions and phenotypic methods for antibiotic resistance. Novel sequence type (ST) was identified in one of the isolates. In total 29 virulence genes were common for all the isolates.

microbiology↗