Biology subjects

Sisobhan, S.

Publications and source records attributed to Sisobhan, S..

2 recordsLinked to original sources

bioRxiv2022

SleepMat: A New Behavioral Analysis Software Program for Sleep and Circadian Rhythms

Drosophila is a premier model for the study of circadian rhythms and sleep, revealing molecular mechanisms that are conserved with vertebrates, including humans. The Drosophila Activity Monitoring (DAM) system has been employed for automated, high throughput analyses, enabling measures of a wide range of measures of circadian and sleep, including period, rhythmicity, anticipation, sleep amount, bout length, and bout number. Yet there are no publicly available programs that capture the full breadth of these measures. Here we develop a new program SleepMat that analyzes a uniquely broader range of circadian and sleep parameters. The program is built on a Matlab platform, employs an easy-to-use graphic user interface, and is highly flexible to customize data inputs. This program will enable a user-friendly high throughput analysis of a broad range of sleep and circadian parameters that can be coupled to the power of Drosophila genetics.

bioinformatics↗

bioRxiv2021

Circadian programming of the ellipsoid body sleep homeostat in Drosophila

Homeostatic and circadian processes collaborate to appropriately time and consolidate sleep and wake. To understand how these processes are integrated, we scheduled brief sleep deprivation at different times of day in Drosophila and find elevated morning rebound compared to evening. These effects depend on discrete morning and evening clock neurons, independent of their roles in circadian locomotor activity. In the R5 ellipsoid body sleep homeostat, we identified elevated morning expression of activity dependent and presynaptic gene expression as well as the presynaptic protein BRUCHPILOT consistent with regulation by clock circuits. These neurons also display elevated calcium levels in response to sleep loss in the morning, but not the evening consistent with the observed time-dependent sleep rebound. These studies reveal the circuit and molecular mechanisms by which discrete circadian clock neurons program a homeostatic sleep center.

neuroscience↗