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Sikora, K. M.

Publications and source records attributed to Sikora, K. M..

2 recordsLinked to original sources

TO BE OR NOT TO BE A PI: ADVICE FOR TRAINEES IN THE BIOMEDICAL/BASIC SCIENCES

When a biomedical or basic science doctoral (PhD) student considers whether or not to become a Principal Investigator (PI), they typically base their decisions on anecdotal data from a limited number of faculty mentors. Alternatively, they may look online for guidance and be met with biased opinions rather than unbiased, comprehensive data. We sought to address this gap by anonymously surveying active faculty at the University of Colorado Anschutz Medical Campus (CU Anschutz) to gain more clarity on the daily life of a PI, the decisions they made as they navigated their career path, and which skills were valuable for securing a faculty position. We also identified important factors in choosing a postdoctoral lab. We found that the majority of PIs would recommend becoming a PI and thought positively of the career path, with most feeling content. But attitudes differed between faculty of different ranks. Further, in addition to scientific vision, hard work, and publications, luck was also deemed as an important factor in becoming and succeeding as a PI. We present our findings so that they may serve as a resource for graduate trainees who are considering a PI/academic research career path. In addition, the information may guide future training plans to optimize achievement of their dream career.

scientific communication and education↗

Differential gene expression in populations of sympathetic neurons purified from a neuropeptide Y reporter mouse

Using gene chip methodology, we identified candidate genes differentially expressed by vasomotor and non-vasomotor sympathetic neurons in the superior cervical ganglion. Groups of 10 neurons were manually sorted after isolation from a transgenic neuropeptide Y (NPY) reporter mouse that expresses the humanized Renilla green fluorescent protein (GFP) under control of Npy promoter sequences. Anatomical analysis of GFP and NPY co-expression showed that 98% of GFP-positive neurons and 16% of GFP-negative neurons express NPY. The probability of contamination in sorted cell samples was therefore high. To minimize this problem, we screened amplified cDNA samples by the quantitative polymerase chain reaction. This approach identified 101 candidate genes preferentially expressed by GFP-positive neurons and 74 candidate genes preferentially expressed by GFP-negative neurons.

neuroscience↗