Biology subjects

Shokrgozar, M. A.

Publications and source records attributed to Shokrgozar, M. A..

4 recordsLinked to original sources

bioRxiv2024

Investigation and optimization the effect of electrical stimulation parameters on the differentiation of human adipose mesenchymal stem cells into neurons-like cells on carbon nanofibers

BackgroundNeurodegenerative diseases are among the most challenging diseases because neuron cells are not able to regenerate spontaneously. Tissue engineering is one of the most promising stem cell-based therapies. Controlling stem cell differentiation is a very crucial aspect of tissue engineering. MethodsIn this study, carbon nanofibers with an average diameter of 181{+/-}45 nm were prepared as a conductive scaffold based on the electrospinning method and subsequent thermal processing. Scaffold structure characterization were performed with XRD, Raman and Electrical conductivity tests. A homemade device was prepared to transmit electrical current to cells seeded on the scaffold in a culture plate. Various current parameters such as current intensity, frequency, waveform, daily shock duration, and shock period on adipose mesenchymal stem cells were examined for differentiation into neuronal cells. SPSS software and the one-way analysis of variance (ANOVA) was used as statistical analysis. ResultsCharacterization tests confirmed the formation of the carbon and crystallite structure with the electrical conductivity . Current with 1500 uA intensity, 500Hz frequency, and square waveform were selected as the optimal current parameters. It was found that the daily and periodic increase in shock time leads to an increase in the expression of neural and glial genes. A comparison of groups with real-time PCR and immunofluorescence of nestin, Map2, TubB3, and GFAPgenes was evaluated. ConclusionsThere are a variety of chemical and physical methods to control cell behavior, one of which is electrical stimulation. Conductive scaffolding is required for direct electrical stimulation of cells. The results showed that the method based on electrical stimulation can well cause neural differentiation, and considering the problems in preparing and maintaining chemical differentiation agents, it can be used practically. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=126 SRC="FIGDIR/small/593090v1_ufig1.gif" ALT="Figure 1"> View larger version (32K): org.highwire.dtl.DTLVardef@159d415org.highwire.dtl.DTLVardef@72bbb3org.highwire.dtl.DTLVardef@811222org.highwire.dtl.DTLVardef@1cda185_HPS_FORMAT_FIGEXP M_FIG O_FLOATNOGraphical abstract:C_FLOATNO stages of scaffold preparation and electrical stimulation of stem cells C_FIG

bioengineering↗

bioRxiv2023

The chicken chorioallantoic membrane model for isolation of CRISPR/cas9-based HSV-1 mutant expressing tumor suppressor p53

Oncolytic viruses (OVs) have emerged as a novel cancer treatment modality, which selectively target and kill cancer cells while sparing normal ones. Among them, engineered Herpes Simplex Virus type 1 has been proposed to be employed as a potential treatment of cancer and was moved to phase III clinical trials. In this study, to improve oncoselectivity and oncotoxicity properties, the UL39 gene of the ICP34.5 deleted HSV-1 was manipulated with the insertion of the EGFP-p53 expression cassette utilizing CRISPR/ Cas9-mediated editing genome. The {Delta}UL39/{Delta}{gamma}34.5/HSV1-p53 mutant was isolated using the chorioallantoic membrane (CAM) of fertilized chicken eggs as a complementing membrane to support the growth of the viruses with gene deficiencies. Phenotypic characterization of {Delta}UL39/{Delta}{gamma}34.5/HSV1-p53-infected cells was compared with the parent {Delta}{gamma}34.5/HSV-1 in vitro. Our results indicate that the CAM model can be a promising strategy for isolating recombinant virus such as HSV-1-P53 that is unable to replicate in cell lines due to the death induced by exogenous p53 during virus replication.

microbiology↗

bioRxiv2022

Carbon nanofibers fabrication, surface modifications, and application as the innovative substrate for electrical stimulation of neural cell differentiation

Engineered nanostructures are innovative and precisely designed, synthesized, and tailored with outstanding physicochemical properties that can be applied as the game-changer in neural tissue engineering. The present study aimed to develop an innovative approach based on electrical stimulation through a conductive scaffold to differentiate neural cells from human adipose mesenchymal stem cells without the use of a specific environment for neural differentiation. Electrospun carbon nanofibers (CNFs) were obtained using heat treatment of polyacrylonitrile nanofibers and treated by nitric acid, ethylenediamine, and oxygen Plasma. SEM imaging revealed that the treated nanofibers have s diameter in the range of 120-200 nm and the treatment did not significantly change the CNFs diameter. The FTIR results showed that the treatments were able to introduce COOH, OH, and NH2 functional groups on the CNFS surface. The XRD and Raman analysis showed that the plasma treatment induced the lowest structural changes in the CNFs microstructure. The biocompatibility assessments showed that the pristine and treated CNFs were non-toxic induced proliferative effect on human adipose-derived mesenchymal stem cells. The electrical stimulation (1.5 mA current with a frequency of 500 Hz and CMOS waveform for 7 days 10 min each day) induced the expression of neural genes and proteins by the cells cultured on the treated CNFs. The Plasma-treated CNFs mediated the highest differentiation outcome. These results indicate that electrospun CNFs can be applied as the innovative interface applicable for neural tissue regeneration under electrical stimulation. Research highlightsO_LICNFs were fabricated from PAN nanofibers C_LIO_LIDifferent amounts of ZnONPs were incorporated into or sprayed on CNF C_LIO_LIincreasing in ZnONP amount decreased conductivity, surface wettability was improved by [~]19-33%. C_LIO_LIAlso, FTIR, XRD, and Raman analyses proved that the presence of ZnONP improved structure formation with lower defect density C_LI O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=96 SRC="FIGDIR/small/512333v1_figS1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@a6eb07org.highwire.dtl.DTLVardef@9040e8org.highwire.dtl.DTLVardef@955840org.highwire.dtl.DTLVardef@d4fa0e_HPS_FORMAT_FIGEXP M_FIG O_FLOATNOSchematic 1.C_FLOATNO The applied electrical stimulation setup C_FIG

bioengineering↗

bioRxiv2020

Simple and Robust Fabrication and Characterization of Conductive Carbonized Nanofibers Loaded with Gold Nanoparticles for Bone Tissue Engineering Applications

Bone tissue engineering is a new and applicable emerging approach to repair the bone defects. Electrical conductive scaffolds through a physiologically relevant physical signaling, electrical stimulation, has shown a highly promise in this approach. In this paper, we fabricated carbon nanofiber/gold nanoparticle (CNF/GNP) conductive scaffolds using two distinct methods; blending electrospinning in which GNP were blended with electrospinning solution, and electrospinning/electrospraying in which GNP was electrosprayed simultaneously with electrospinning. The obtained electrospun mats underwent stabilization/carbonization process. The scaffolds were characterized by SEM, XRD, FT-IR and Raman spectroscopy. SEM characterizations showed improved morphology and a slight decrease in the diameter of the spinned and sprayed nanofibers (from 178.66 {+/-} 38.40 nm to 157.94 {+/-} 24.14 nm and 120.81 {+/-} 13.77 nm, respectively), while XRD analysis confirmed the crystal structure of the nanofibers. Raman spectroscopy revealed enhancement in the graphitization of the structure, and the electrical conductivity of the structure improved by up to 29.2% and 81% in electrospraying and blending electrospinning modes, respectively. Indirect MTT and LDH toxicity assays directly were performed to assess MG63 cell toxicity, but no significant toxicity was observed and the scaffolds did not adversely affect cell proliferation. It can be concluded this structure have potential for bone tissue engineering applications. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=80 SRC="FIGDIR/small/013383v2_ufig1.gif" ALT="Figure 1"> View larger version (28K): org.highwire.dtl.DTLVardef@1ac84f1org.highwire.dtl.DTLVardef@14bef2dorg.highwire.dtl.DTLVardef@438261org.highwire.dtl.DTLVardef@24e1b4_HPS_FORMAT_FIGEXP M_FIG C_FIG

bioengineering↗