Biology subjects

Shimogawa, M. M.

Publications and source records attributed to Shimogawa, M. M..

4 recordsLinked to original sources

bioRxiv2025

CryoEM structure of the central apparatus of the Trypanosoma brucei flagellum

Axonemes are cylindrical bundles of microtubule filaments that typically follow a 9+n pattern (where n ranges from 0 to 4). However, variations exist across species and cell types, including architectures with fewer (e.g., 3+0, 6+0) or more than nine doublet microtubules (e.g., 9+9+0, 9+9+3), reflecting diverse structural adaptations of cilia and flagella in eukaryotes. Trypanosoma brucei, the causative agent of African trypanosomiasis, relies on its single 9+2 flagellum to navigate through environments within the mammalian host and insect vector. Central to the T. brucei flagellums function is a canonical central apparatus (CA), composed of two--C1 and C2-- singlet microtubules, which regulates flagellar beating and ensures efficient movement. Despite its crucial mechanoregulatory role in flagellar beating, the molecular structure and interactions governing T. brucei CA assembly and function remain poorly understood. In this study, we employed cryogenic electron microscopy (cryoEM) to uncover structural details of the T. brucei CA. We identified conserved and stably C1/C2-associated protein densities, including the armadillo repeat protein PF16, which serves as a structural scaffold critical for CA assembly and axonemal asymmetry. Our analysis also revealed pronounced molecular flexibility of the CA and uncovered T. brucei-specific densities, suggesting lineage-specific adaptations for parasite motility. These findings provide critical insights into the structural foundations of T. brucei motility. They also highlight potential therapeutic targets to disrupt the parasites ability to cause disease, offering new avenues for the treatment of African trypanosomiasis. Comparison of CAs in this canonical 9+2 axoneme and non-canonical 9+n axonemes offers general insights into the assembly and diverse functions of CAs across a wide range of species. SignificanceThe flagellum of Trypanosoma brucei, the parasite causing African trypanosomiasis, drives motility essential for host infection and disease transmission. Our cryogenic electron microscopy study reveals the molecular architecture of its central apparatus, identifying PF16 as a key scaffold that stabilizes axonemal asymmetry and imparts flexibility critical for flagellar beating. We uncover conserved proteins and T. brucei- specific adaptations, highlighting evolutionary divergence underlying the parasites distinctive motility. These structural results offer insights about how the central apparatus regulates T. bruceis bihelical motion and unveil potential therapeutic targets to disrupt flagellar function and combat African trypanosomiasis. Comparison among the central apparatus of canonical and non-canonical axonemes allow us to suggest broader principles underlying their central roles in ciliary functions across eukaryotes.

microbiology↗

bioRxiv2024

FAP20 is required for flagellum assembly in Trypanosoma brucei

Trypanosoma brucei is a human and animal pathogen that depends on flagellar motility for transmission and infection. The trypanosome flagellum is built around a canonical "9+2" axoneme, containing nine doublet microtubules (DMTs) surrounding two singlet microtubules. Each DMT contains a 13-protofilament A-tubule and a 10-protofilament B-tubule, connected to the A-tubule by a conserved, non-tubulin inner junction (IJ) filament made up of alternating PACRG and FAP20 subunits. Here we investigate FAP20 in procyclic form T. brucei. A FAP20-NeonGreen fusion protein localized to the axoneme as expected. Surprisingly, FAP20 knockdown led to a catastrophic failure in flagellum assembly and concomitant lethal cell division defect. This differs from other organisms, where FAP20 is required for normal flagellum motility, but generally dispensable for flagellum assembly and viability. Transmission electron microscopy demonstrates failed flagellum assembly in FAP20 mutants is associated with a range of DMT defects and defective assembly of the paraflagellar rod, a lineage-specific flagellum filament that attaches to DMT 4-7 in trypanosomes. Our studies reveal a lineage-specific requirement for FAP20 in trypanosomes, offering insight into adaptations for flagellum stability and motility in these parasites and highlighting pathogen versus host differences that might be considered for therapeutic intervention in trypanosome diseases. SIGNIFICANCE STATEMENTO_LIDiverse eukaryotic organisms rely on a generally conserved axoneme architecture and dynein-dependent beating mechanism to drive motility, but mechanisms conferring lineage-specific motility needs are largely unknown. C_LIO_LIFAP20 is a conserved flagellar protein that impacts flagellum motility in multiple organisms. C_LIO_LIThe current work demonstrates FAP20 is particularly important in the pathogen, T. brucei, providing insight into pathogen adaptations for moving through host environments and illuminating targets to consider for therapeutic intervention in trypanosome diseases. C_LI

cell biology↗

bioRxiv2022

FAP106 is an interaction hub required for stable assembly of conserved and lineage-specific proteins at the cilium inner junction

Motility of pathogenic protozoa depends on flagella (synonymous with cilia) with axonemes containing nine doublet microtubules (DMTs) and two singlet microtubules. Microtubule inner proteins (MIPs) within DMTs influence axoneme stability and motility and provide lineage-specific adaptations, but individual MIP functions and assembly mechanisms are mostly unknown. Here, we show in the sleeping sickness parasite Trypanosoma brucei, that FAP106, a conserved MIP at the DMT inner junction, is required for trypanosome motility and functions as a critical interaction hub, directing assembly of several conserved and lineage-specific MIPs. We further use comparative cryogenic electron tomography (cryoET) and quantitative proteomics to identify novel MIP candidates, and RNAi knockdown plus fitting of AlphaFold models into cryoET maps to demonstrate one of these, MC8, is a trypanosome-specific MIP required for parasite motility. Our work advances understanding of MIP assembly mechanisms and identifies lineage-specific motility proteins that are attractive targets to consider for therapeutic intervention.

cell biology↗

bioRxiv2020

APEX2 proximity proteomics resolves flagellum subdomains and identifies flagellum tip-specific proteins in Trypanosoma brucei

Trypanosoma brucei is the protozoan parasite responsible for sleeping sickness, a lethal vector-borne disease. T. brucei has a single flagellum that plays critical roles in parasite biology, transmission and pathogenesis. An emerging concept in flagellum biology is that the organelle is organized into subdomains, each having specialized composition and function. Overall flagellum proteome has been well-studied, but a critical gap in knowledge is the protein composition of individual flagellum subdomains. We have therefore used APEX-based proximity proteomics to examine protein composition of T. brucei flagellum subdomains. To assess effectiveness of APEX-based proximity labeling, we fused APEX2 to the DRC1 subunit of the nexin-dynein regulatory complex, an axonemal complex distributed along the flagellum. We found that DRC1-APEX2 directs flagellum-specific biotinylation and purification of biotinylated proteins yields a DRC1 "proximity proteome" showing good overlap with proteomes obtained from purified axonemes. We next employed APEX2 fused to a flagellar membrane protein that is restricted to the flagellum tip, adenylate cyclase 1 (AC1), or a flagellar membrane protein that is excluded from the flagellum tip, FS179. Principal component analysis demonstrated the pools of biotinylated proteins in AC1-APEX2 and FS179-APEX2 samples are distinguished from each other. Comparing proteins in these two pools allowed us to identify an AC1 proximity proteome that is enriched for flagellum tip proteins and includes several proteins involved in signal transduction. Our combined results demonstrate that APEX2-based proximity proteomics is effective in T. brucei and can be used to resolve proteome composition of flagellum subdomains that cannot themselves be readily purified. IMPORTANCESleeping sickness is a neglected tropical disease, caused by the protozoan parasite Trypanosoma brucei. The disease disrupts the sleep-wake cycle, leading to coma and death if left untreated. T. brucei motility, transmission, and virulence depend on its flagellum (aka cilium), which consists of several different specialized subdomains. Given the essential and multifunctional role of the T. brucei flagellum, there is need of approaches that enable proteomic analysis of individual subdomains. Our work establishes that APEX2 proximity labeling can, indeed, be implemented in the biochemical environment of T. brucei, and has allowed identification of proximity proteomes for different subdomains. This capacity opens the possibility to study the composition and function of other compartments. We further expect that this approach may be extended to other eukaryotic pathogens, and will enhance the utility of T. brucei as a model organism to study ciliopathies, heritable human diseases in which cilia function is impaired.

microbiology↗