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Shchukovskaia, A.

Publications and source records attributed to Shchukovskaia, A..

2 recordsLinked to original sources
bioRxiv2021

Assessment of the suitability of marker fragments DNA sequences of the internal transcribed spacer region (ITS), beta-tubulin gene (TUB) and elongation factor (EF1) genes for diagnostics of Fusarium tricinctum species complex in Russian in grain products

By results of laboratory researches from plant samples of grain crops species Fusarium avenaceum, Fusarium tricinctum and Fusarium acuminatum related to Fusarium tricinctum species complex were isolated and identified, based on cultural and morphological characteristics, molecular-genetic analysis of 3 DNA fragments was performed: internal transcribed spacer (ITS) site, beta-tubulin gene site (TUB), elongation factor gene site (EF1) showed insignificant intraspecific and interspecific variability at ITS and EF1 sites, which may negatively affect the reliability of target species identification, TUB site was more effective for distinguishing and identifying the studied species, due to significant intraspecific and interspecific variability

molecular biology↗
bioRxiv2021

Potential possibility of using the DNA regions of the internal transcribed spacer ITS and the beta-tubulin gene TUB in the diagnosis of the pathogen Mycosphaerella linicola Naumov.

During the study, the suitability of the ITS and TUB DNA regions was shown for identifying the target species at the genetic level. However, these DNA regions do not reveal significant differences between the sequences of the target species from different geographic regions. However, when constructing dendrograms of similarities and differences, the sequential differentiation between isolates at the population level was found, which may further affect the results of identification of the target species.

molecular biology↗