Biology subjects

Shashkina, S. S.

Publications and source records attributed to Shashkina, S. S..

3 recordsLinked to original sources

bioRxiv2025

Accumulation of ibuprofen in endemic amphipods of Lake Baikal

Pharmaceutical pollutants, including ibuprofen, are now ubiquitously detected in global aquatic ecosystems, exerting significant negative ecological impacts. Lake Baikal organisms have been documented to accumulate ibuprofen and related contaminants. This study quantified ibuprofen concentrations within Lake Baikals endemic amphipod fauna using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). We analyzed specimens representing key ecological groups across the genera Eulimnogammarus, Brandtia, Ommatogammarus, and Pallasea. Ibuprofen concentrations ranged from 4.19 ng/g to 1151.32 ng/g (wet weight), confirming consistent contamination in both littoral and deep-water endemic amphipod populations. Crucially, our data provide the first evidence suggesting amphipods, or their associated symbiotic microbiota, may metabolize ibuprofen. Interspecific accumulation patterns were identified, with Eulimnogammarus sp. and Brandtia sp. exhibiting distinct profiles. Furthermore, accumulation was significantly higher during spring compared to autumn samples. A negative correlation emerged between ibuprofen concentration and amphipod body mass within species. Several populations contained non-detectable levels. These findings demonstrate that endemic amphipods within Lake Baikals natural environment are exposed to and bioaccumulate the pharmaceutical pollutant ibuprofen, exhibiting species-specific, seasonal, and allometric variation in uptake.

ecology↗

bioRxiv2024

Effect of truffle extracts on growth of Chlorella vulgaris

Truffles are hypogeous ectomycorrhizal fungi with a complex system of chemical communications and biotic interactions. This study examines the influence of truffle extracts on the growth of the alga Chlorella vulgaris as a model organism commonly employed in pharmacology, food industry and biomonitoring. The study involved sampling of fruiting bodies of black truffles in a number of regions across Russia, over a number of years. The observed differences in truffle condition were used to illustrate a potential correlation between the quality and biological activity of truffles. Methanolic extracts were prepared and added to the culture of C. vulgaris to evaluate the growth of algae using the spectrophotometry methods. For the first time, we demonstrated that truffle extracts had biological activity in terms of the algae C. vulgaris growth stimulation. We observed the effects of short-term and long-term growth stimulation. To date, it can be concluded that there is no direct correlation between the stimulating effects on algae and the state of truffles, place of their sampling, or their quality. Finally, it is crucial to examine biological regulations that operate in complex systems such as truffles. It is suggested that in the near future truffles may become a model system for the study of complex biotic and chemical communications.

biochemistry↗

bioRxiv2024

Detection of pharmaceutical contamination in amphipods of Lake Baikal by the HPLC-MS method

Pollution by active ingredients is one of the most significant and widespread forms of pollution on Earth. Medicines can have a negative impact on ecosystems, and contamination can have unpredictable consequences. An urgent and unexplored task is to study the Lake Baikal ecosystem and its organisms for the presence of trace concentrations of active pharmaceutical ingredients. Our study aimed to conduct a qualitative analysis of active pharmaceutical ingredients, and quantitative analysis of ibuprofen in endemic amphipods of Lake Baikal, using methods of high-performance liquid chromatography and mass spectrometry (HPLC-MS). Acetylsalicylic acid (aspirin), ibuprofen, acetaminophen, azithromycin, dimetridazole, metronidazole, amikacin, spiramycin, and some tetracycline antibiotics were detected in the studied littoral amphipods. We also detected different annual loads of active pharmaceutical ingredients on amphipods. Using the multiple reaction monitoring (MRM) mode mentioned in GOST International Technical Standards, we detected molecules, fragmented as amikacin, chlortetracycline, doxycycline, oxytetracycline, dimetridazole, metronidazole and spiramycin. Thus, we first revealed that invertebrates of Lake Baikal can encounter pharmaceutical contaminants in the environment.

ecology↗