Biology subjects

Sharaby, Y.

Publications and source records attributed to Sharaby, Y..

2 recordsLinked to original sources

bioRxiv2024

Redox-driven control of Yuh1/UCHL3 impacts mitochondrial health via NEDD8/Rub1 pathway

The ubiquitin-like protein NEDD8/Rub1 undergoes processing by the enzyme Yuh1/UCHL3 to become functional. While the processed NEDD8/Rub1 modifies Cullin-RING E3 ligases (CRLs) among all studied organisms, its role in facilitating CRL-based substrate degradation is absent in S. cerevisiae. This prompts questions about NEDD8/Rub1 functionality if it does not activate CRLs universally. Previous studies revealed that increased production of reactive oxygen species (ROS) during the glycolysis to mitochondrial respiration transition inhibits cullin NEDDylation in S. cerevisiae, yet the specific affected enzymes remain unidentified. Here, we investigate how redox changes affect Yuh1 activity, revealing a thiol-based redox switch modulating its catalytic function in response to ROS. Temporal inactivation of Yuh1 fine-tunes NEDD8/Rub1 mature and precursor species, both crucial for maintaining mitochondrial integrity and enhancing oxidative stress resilience. These findings unveil a novel role for Rub1/NEDD8 beyond CRL activation, linking redox signaling to NEDD8/Rub1 pathways. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=137 SRC="FIGDIR/small/594945v1_ufig1.gif" ALT="Figure 1"> View larger version (28K): org.highwire.dtl.DTLVardef@1ce0c41org.highwire.dtl.DTLVardef@8690bdorg.highwire.dtl.DTLVardef@8898c6org.highwire.dtl.DTLVardef@1e61a85_HPS_FORMAT_FIGEXP M_FIG C_FIG

cell biology↗

bioRxiv2021

Wild Wheat Introgression Promotes Temporal Water fluxes Dynamics under Terminal Drought Stress through Plant-Atmospheric Interrelations

Drought intensity as experienced by plants depends upon soil moisture status and atmospheric variables such as temperature, radiation, and air vapour pressure deficit (VPD). Although the role of shoot architecture with these edaphic and atmospheric factors is well-characterized, the extent to which shoot and root dynamic interactions as a continuum are controlled by genotypic variation is less known. Here, we targeted these interactions using a wild emmer introgression line (IL20) with a distinct drought-induced shift in the shoot-to-root ratio and its drought-sensitive recurrent parent Svevo. Using a gravimetric platform, we show that IL20 maintained higher root water influx and gas exchange under drought stress, which supported a greater growth. Interestingly, the advantage of IL20 in root water influx and transpiration was expressed earlier during the daily diurnal cycle under lower VPD and therefore supported higher transpiration efficiency. Application of structural equation model indicates that under drought, VPD and radiation are antagonistic to transpiration rate, whereas the root water influx operates as feedback for the higher atmospheric responsiveness of leaves. Collectively, our results suggest that a drought-induced shift in root-to-shoot ratio can improve plant water uptake potential in a short preferable time window determined by both water and atmospheric parameters.

plant biology↗