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Shafer, A. B. A.

Publications and source records attributed to Shafer, A. B. A..

14 recordsLinked to original sources

Epigenetic clocks and nonlinear aging in Odocoileus deer

Population age structure has important consequences for population dynamics and is a key parameter for wildlife demography and management. Epigenetic clocks have emerged as accurate molecular tools for estimating chronological age. Ungulates are among the most intensively managed wildlife worldwide, yet epigenetic clocks have been developed for only two cervid species. Here, we develop a novel epigenetic clock for mule deer (Odocoileus hemionus) and a multi-species clock for the Odocoileus genus, and use them to investigate variation in age-associated DNA methylation across the mule deer lifespan. The mule deer clock achieved accuracy comparable to recently developed mammalian epigenetic clocks (r {approx} 0.9; MAE {approx} 5% of species lifespan). The multi-species Odocoileus clock showed lower predictive performance (r = 0.72; MAE = 0.782 years), but its broader taxonomic applicability may be particularly valuable where Odocoileus species occur sympatrically and hybridize. Cross-sectional epigenetic age estimates supported nonlinear age-associated methylation dynamics across the mule deer lifespan, whereas longitudinal changes in epigenetic age indicated that clocks optimized for cross-sectional age estimation may lack sufficient resolution to quantify short-term within-individual changes. Overall, our results highlight the value of integrating clock-level, CpG-level, and longitudinal analyses to investigate aging in wild populations. We also identify strongly age-associated loci that provide candidates for developing targeted, scalable, and lower-cost assays.

molecular biology↗

Spatial Variability in Soil Necrobiome Communities has a Negligible Effect on Postmortem Interval Estimation

Microbial succession can be used to estimate the postmortem interval (PMI); however, the impact of spatial variability within the cadaver decomposition island (CDI) is not well understood. This study examined spatial variation in necrobiome communities where soil samples were collected over time and across spatial locations from the CDIs of two human body donors. Microbial communities were characterized using 16S rRNA sequencing and statistical modelling of variation and PMI were conducted. Necrobiome community metrics showed no significant differences across anatomical sampling sites within the CDI at a single timepoint. Temporal modelling identified 11 taxa with significant relationships to PMI in one donor, with spatial sampling having a minimal impact on the PMI relationships. Non-linear approaches also identified taxa with likely PMI signals in the second donor. These findings demonstrate that opportunistic sampling can capture robust linear and non-linear PMI signals in later decomposition stages.

microbiology↗

Ancient DNA perspectives on North American cervids identify dispersal waves and fluctuations in diversity

North American fauna has experienced major changes over the last two million years. Glacial cycles, animal migrations, peopling waves, and, more recently, European colonisation, caused demographic fluctuations, divergence, and wildlife extinctions. Caribou (Rangifer tarandus), moose (Alces alces), wapiti (Cervus canadensis), white-tailed (Odocoileus virginianus) and mule deer (O. hemionus) are the five extant cervids in Northern America, with unique dispersal patterns and conservation statuses. Here, we used DNA analysis of ancient, historical and modern individuals of these Cervidae to retrace evolutionary processes through time. We show that present-day eastern Canada caribou share ancestry with a 30-thousand-year-old sample from western Canada consistent with the species' complex dispersal history as suggested by literature. Our results suggest rapid expansion of moose following their arrival on the continent ~15 thousand years ago. Data on wapiti shows a recent loss of diversity consistent with its large-scale extirpation after European colonisation. Results on Odocoileus species are in line with their recognised mito-nuclear discordance. Our results exemplify the utility of chronological sampling to gain a better understanding of how external pressures drive genomic changes.

evolutionary biology↗

Allopatric speciation in cattails: Genomic landscapes of divergence across Typha spp. suggest balancing selection, introgressions, and the absence of adaptive divergence

Understanding how speciation unfolds is a central goal of evolutionary biology. Historically, global climatic fluctuations have triggered allopatric diversification. Using genome-wide data, we reconstructed the phylogenetic relationships and demographic histories of five Typha species, a plant genus foundational to freshwater ecosystems, with widespread, partially sympatric ranges and at least one widespread, regionally invasive hybrid zone (Typha x glauca). Molecular clock and demographic analyses indicate that the species in this study diverged in the absence of gene flow, during periods roughly contemporaneous with population bottlenecks and expansions in Typha--likely triggered by geoclimatic events--and that this divergence was followed by secondary contact in more recent times. Genomic scans showed no evidence of selection with gene flow driving species differentiation, suggesting a minor role for sympatric ecological speciation underlying divergence. These observations are consistent with expectations for drift-driven allopatric speciation. Allopatric speciation in T. latifolia and T. angustifolia, their low genetic differentiation, and the scarcity of ecological divergence in building their reproductive isolation could explain these species ability to hybridise.

evolutionary biology↗

Comparative genomics of the world's smallest mammals reveals links to echolocation, metabolism, and body size plasticity

Originating 30 million years ago, shrews (Soricidae) have diversified into around 400 species worldwide. Shrews display a wide array-array of adaptations, with some species having developed distinctive traits such as echolocation, underwater diving, and venomous saliva. Accordingly, these tiny insectivores are ideal to study the genomic mechanisms of evolution and adaptation. We conducted a comparative genomic analysis of four shrew species and 16 other mammals to identify genomic variations unique to shrews. Using two existing shrew genomes and de novo assemblies for the maritime (Sorex maritimensis) and smoky shrew (S. fumeus), we identified mutations in conserved regions of the genomes, also known as accelerated regions, gene families undergoing significant expansion, and positively selected genes. Our analyses unveiled shrew-specific genomic variants in genes associated with the nervous, metabolic, and auditory systems, which can be linked to unique traits in shrews. Notably, genes suggested to be under convergent evolution in echolocating mammals exhibited accelerated regions in shrews, and pathways linked to putative body size plasticity were detected. These findings provide insight into the evolutionary mechanisms shaping shrew species, shedding light on their adaptation and divergence over time.

genomics↗

Epigenetic clocks, sex markers, and age-class diagnostics in three harvested large mammals

The development of epigenetic clocks, or the DNA methylation-based inference of age, is an emerging tool for ageing in free ranging populations. In this study, we developed epigenetic clocks for three species of large mammals that are the focus of extensive management throughout their range: white-tailed deer, black bear, and mountain goat. We quantified differential DNA methylation patterns at over 30,000 cytosine-guanine sites (CpGs) from tissue samples (N=141) of all three species. We used a penalized regression model (elastic net) to build highly explanatory (black bear r = 0.95; white-tailed deer r = 0.99; mountain goat r = 0.97) and robust (black bear Median Absolute Error or MAE = 1.33; white-tailed deer MAE = 0.29; mountain goat MAE = 0.61) models of age (clocks). We also characterized individual CpG sites within each species that demonstrated clear differences in methylation levels between age classes and sex, which can be used to develop a suite of accessible diagnostic markers. Our results demonstrate promising tools for the large-scale estimation of age in wild animals, which have the potential to contribute to wildlife monitoring by providing easily obtainable representations of age structure in managed populations.

genomics↗

Genomic correlates for migratory direction in a free-ranging cervid

Animal migrations are some of the most ubiquitous and one of the most threatened ecological processes. A wide range of migratory behaviours occur in nature, and this behaviour is not uniform between and within species, where even individuals in the same population can exhibit differentiation. While the environment largely drives migratory behaviour, it is necessary to understand the genetic mechanisms influencing migration to elucidate the adaptive potential of migratory species to cope with novel conditions and adapt to environmental change. In this study, we identified genes associated with migratory traits by undertaking pooled genome-wide scans on a natural population of a facultatively migrating ungulate. We identified genomic regions associated with variation in migratory direction in the study population, including FTM1, a gene linked to the formation of lipids, and DPPA3, a gene linked to epigenetic modifications of the maternal line. Such a genetic basis for migratory traits can have implications on heritability of behaviour and the flexibility of individuals to change their behaviour in the face of stochastic changes in their environment.

genomics↗

Differential methylation, epigenetic clocks, and island-mainland divergence in an insectivorous small mammal

Geographically isolated populations, specifically island-mainland counterparts, tend to exhibit phenotypic variation in many species. The so-called island syndrome occurs when different environmental pressures lead to insular divergence from mainland populations. This phenomenon can be seen in an island population of Nova Scotia masked shrews (Sorex cinereus), which have developed a specialized feeding habit and digestive enzyme compared to their mainland counterparts. Epigenetic modifications, such as DNA methylation (DNAm), can impact phenotypes by altering gene expression without changing the DNA sequence. Here, we used a de novo masked shrew genome assembly and a mammalian methylation array profiling 37 thousand conserved CpGs to investigate morphological and DNA methylation patterns between island and mainland populations. Island shrews were morphologically and epigenetically different than their mainland counterparts, exhibiting a smaller body size. A gene ontology enrichment analyses of differentially methylated CpGs implicated developmental and digestive system related pathways. Based on our shrew epigenetic clock, island shrews might also be aging faster than their mainland counterparts. This study provides novel insight on phenotypic and epigenetic divergence in island-mainland mammal populations and suggests an underlying role of methylation in island-mainland divergence.

genomics↗

Genome assembly, annotation and comparative analysis of the cattail Typha latifolia

Cattails (Typha species) comprise a genus of emergent wetland plants with a global distribution. Typha latifolia and T. angustifolia are two of the most widespread species, and in areas of sympatry can interbreed to produce the hybrid Typha x glauca. In some regions the relatively high fitness of T. x glauca allows it to outcompete and displace both parent species, while simultaneously reducing plant and invertebrate biodiversity, and modifying nutrient and water cycling. We generated a high-quality whole genome assembly of T. latifolia using PacBio long-read and high coverage Illumina sequences that will facilitate evolutionary and ecological studies in this hybrid zone. Genome size was 287 Mb and consisted of 1,189 scaffolds, with an N50 of 8.706 Mb; 43.84% of the genome were identified as repetitive elements. The assembly has a BUSCO score of 96.03%, and 27,432 genes and 2,700 RNA sequences were putatively identified. Comparative analysis detected over 9,000 shared orthologues with related taxa and phylogenomic analysis supporting Typha latifolia as a divergent lineage within Poales. This high-quality scaffold-level reference genome will provide a useful resource for future population genomic analyses and improve our understanding of Typha hybrid dynamics.

genomics↗

A diagnostic relationship between the RNA Integrity Number equivalent and Time Since Deposition of blood

Determining the time since deposition (TSD) of bloodstains would provide forensic scientists with critical information regarding the timeline of the events involving bloodshed. The physicochemical changes occurring to biomolecules as a bloodstain degrades can be used to approximate the TSD of bloodstains. Our study aims to quantify the timewise degradation trends and temperature dependence found in total RNA from bloodstains without the use of amplification, expanding the scope of the RNA TSD research which has previously targeted mRNA molecules. Whole bovine blood was stored in plastic microcentrifuge tubes at 21{degrees}C or 4{degrees}C and tested over different timepoints spanning one week. Nine RNA metrics were visually assessed and quantified using linear and mixed models; the RNA Integrity Number equivalent (RINe) and the DV200 demonstrated strong negative trends over time and statistical independence. The RINe model fit was high (R2 = 0.60), and while including the biological replicate as a random effect increased the fit for all RNA metrics, no significant differences were found between biological replicates stored at the same temperature for the RINe and DV200 metrics. Importantly, this suggests that these standardized metrics can likely be directly compared between scenarios and individuals, with DV200 having an inflection point at [~]28 hrs. This study provides a novel approach for blood TSD estimates, producing metrics that are not affected by inter-individual variation and improving our understanding of the rapid degradation occurring in bloodstains. HIGHLIGHTSO_LIAmplification-free analysis of total RNA in degrading bloodstains. C_LIO_LIShort-term RNA degradation assessment using high-resolution size measurements. C_LIO_LITotal RNA quality and quantity metrics were assessed across a one-week. C_LIO_LITotal RNA quality metrics demonstrated the strongest timewise trends. C_LIO_LIBiological replicates produced similar results for RNA quality metrics. C_LI

genetics↗

Gut microbiome composition predicts summer core range size in a generalist and specialist ungulate

The gut microbiome of animals varies by age, diet, and habitat, and directly influences individual health. Similarly, variation in an individuals home range can lead to differences in feeding strategies and fitness. Ungulates (hooved mammals) exhibit species-specific microbiomes and habitat use patterns: here, we combined gut microbiome and movement data to assess relationships between space use and the gut microbiome in a specialist and a generalist ungulate. We captured and GPS radiocollared 24 mountain goats (Oreamnos americanus) and 34 white-tailed deer (Odocoileus virginianus). We collected fecal samples and conducted high-throughput sequencing of the 16S rRNA gene. Using GPS data, we estimated core (50%) and home range (95%) sizes and calculated proportional use for several important habitat types. We generated metrics related to gut diversity and key bacterial ratios. We hypothesize that larger Firmicutes to Bacteroides ratios confer body size or fat advantages that allow for larger home ranges, and that relationships between gut diversity and disproportionate habitat use is stronger in mountain goats due to their restricted niche relative to white-tailed deer. Firmicutes to Bacteroides ratios were positively correlated with core range area in both species. Mountain goats exhibited a negative relationship between gut diversity and use of two key habitat types (treed areas and escape terrain), whereas no relationships were detected in white-tailed deer. This is the first study to relate core range size to the gut microbiome in wild ungulates and is an important proof of concept that advances the information that can be gleaned from non-invasive sampling.

molecular biology↗

Space, time, and captivity: quantifying factors influencing the microbiome of an alpine ungulate

The community of microorganisms in the gut is affected by host species, diet, and environment and is linked to normal functioning of the host organism. Although the microbiome fluctuates in response to host demands and environmental changes, there are core groups of microorganisms that remain relatively constant throughout the hosts lifetime. Ruminants are mammals that rely on highly specialized digestive and metabolic modifications, including microbiome adaptations, to persist in extreme environments. Here, we assayed the fecal microbiome of four mountain goat (Oreamnos americanus) populations in western North America. We quantified fecal microbiome diversity and composition among groups in the wild and captivity, across populations, and in a single group over time. There were no differences in community evenness or diversity across groups, although we observed a decreasing diversity trend across summer months. Pairwise sample estimates grouped the captive population distinctly from the wild populations, and moderately grouped the southern wild group distinctly from the two northern wild populations. We identified 33 genera modified by captivity, with major differences in key groups associated with cellulose degradation that likely reflect differences in diet. Our findings are consistent with other ruminant studies and provide baseline microbiome data in this enigmatic species, offering valuable insights into the health of wild alpine ungulates.\n\nO_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=184 SRC=\"FIGDIR/small/475459v3_ufig1.gif\" ALT=\"Figure 1\">\nView larger version (8K):\norg.highwire.dtl.DTLVardef@1d5256eorg.highwire.dtl.DTLVardef@8c973forg.highwire.dtl.DTLVardef@1db5b0org.highwire.dtl.DTLVardef@835191_HPS_FORMAT_FIGEXP M_FIG C_FIG SummaryThis study characterizes the microbiome of mountain goat (Oreamnos americanus) populations across populations and over summer months; we also quantified the effects of captivity to offer more insights into the health of alpine wildlife.

microbiology↗

Methylation patterns reveal cryptic structure and a pathway for adaptation in a panmictic carnivore

Determining the molecular signatures of adaptive differentiation is a fundamental component of evolutionary biology. A key challenge remains for identifying such signatures in wild organisms, particularly between populations of highly mobile species that undergo substantial gene flow. The Canada lynx (Lynx canadensis) is one species where mainland populations appear largely undifferentiated at traditional genetic markers, despite inhabiting diverse environments and displaying phenotypic variation. Here, we used high-throughput sequencing to investigate both neutral genetic structure and epigenetic differentiation across the distributional range of Canada lynx. Using a customized bioinformatics pipeline we scored both neutral SNPs and methylated nucleotides across the lynx genome. Newfoundland lynx were identified as the most differentiated population at neutral genetic markers, with diffusion approximations of allele frequencies indicating that divergence from the panmictic mainland occurred at the end of the last glaciation, with minimal contemporary admixture. In contrast, epigenetic structure revealed hidden levels of differentiation across the range coincident with environmental determinants including winter conditions, particularly in the peripheral Newfoundland and Alaskan populations. Several biological pathways related to morphology were differentially methylated between populations, with Newfoundland being disproportionately methylated for genes that could explain the observed island dwarfism. Our results indicate that epigenetic modifications, specifically DNA methylation, are powerful markers to investigate population differentiation and functional plasticity in wild and non-model systems.\n\nSIGNIFICANCEPopulations experiencing high rates of gene flow often appear undifferentiated at neutral genetic markers, despite often extensive environmental and phenotypic variation. We examined genome-wide genetic differentiation and DNA methylation between three interconnected regions and one insular population of Canada lynx (Lynx canadensis) to determine if epigenetic modifications characterized climatic associations and functional molecular plasticity. Demographic approximations indicated divergence of Newfoundland during the last glaciation, while cryptic epigenetic structure identified putatively functional differentiation that might explain island dwarfism. Our study suggests that DNA methylation is a useful marker for differentiating wild populations, particularly when faced with functional plasticity and low genetic differentiation.

genomics↗

Genome Sequence of Indian Peacock Reveals the Peculiar Case of a Glittering Bird

The unique ornamental features and extreme sexual traits of Peacock have always intrigued the scientists. However, the genomic evidence to explain its phenotype are yet unknown. Thus, we report the first genome sequence and comparative analysis of peacock with the available high-quality genomes of chicken, turkey, duck, flycatcher and zebra finch. The candidate genes involved in early developmental pathways including TGF-{beta}, BMP, and Wnt signaling pathway, which are also involved in feather patterning, bone morphogenesis, and skeletal muscle development, showed signs of adaptive evolution and provided useful clues on the phenotype of peacock. The innate and adaptive immune components such as complement system and T-cell response also showed signs of adaptive evolution in peacock suggesting their possible role in building a robust immune system which is consistent with the between species predictions of Hamilton-Zuk hypothesis. This study provides novel genomic and evolutionary insights into the molecular understanding towards the phenotypic evolution of Indian peacock.

genomics↗