Dynamic Magnetic Resonance Imaging of Whole-Stomach Motility in Rats
ObjectiveUnderstanding gastric physiology in rodents is critical for advancing preclinical neurogastroenterology research. However, existing techniques are often invasive, terminal, or limited in resolution. This study aims to develop a non-invasive, standardized MRI protocol capable of capturing whole-stomach dynamics in anesthetized rats with high spatiotemporal resolution. MethodsExperiments were performed in a 7-Tesla MRI system. Gadolinium-doped test meals were prepared to enhance intraluminal contrast in T1-weighted MRI. Based on a modified multi-slice gradient-echo sequence, our protocol integrates respiratory gating to minimize motion artifacts, spatial saturation to improve intraluminal contrast, and slice grouping to optimize the trade-offs between signal-to-noise ratio and motion sensitivity. Image acquisition was accelerated using a time-interleaved k-space undersampling scheme, with missing data reconstructed through k-t interpolation. Image quality and gastric motility were quantitatively assessed. ResultsThe protocol enabled successful imaging of the stomach and visualization of its pseudo-periodic dynamics in anesthetized rats. The gadolinium-doped meal produced relatively homogeneous intraluminal contrast, allowing clear delineation of gastric anatomy, volume, and motility. The retrospectively reconstructed image exhibited high image quality and yielded reliable estimates of antral contractions, confirming the effectiveness and robustness of k-t interpolation method. Estimated antral contraction amplitude and velocity showed minimal deviations from the reference values, whereas contraction frequency estimation remained highly consistent and accurate. Prospective acquisitions using the accelerated imaging protocol successfully imaged the entire stomach and major intestinal regions, acquiring 24 slices every < 3 s and capturing antral contraction at [~]5 cycles per minute. ConclusionWe established an accessible and standardized imaging protocol that encompasses contrast meal preparation, animal handling and training, and a contrast-enhanced dynamic GI MRI acquisition and reconstruction framework. SignificanceThis protocol provides a comprehensive, robust, non-invasive tool for studying gastric motility and dysmotility in rodents, offering strong potential to advance preclinical gastrointestinal motility research. Graphic AbstractIn this paper, we report a standardized, non-invasive imaging protocol that encompasses animal handling and training, contrast meal preparation, and a contrast-enhanced dynamic GI MRI acquisition and reconstruction framework for imaging whole-stomach dynamics in anesthetized rats. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=39 SRC="FIGDIR/small/657757v1_ufig1.gif" ALT="Figure 1"> View larger version (13K): org.highwire.dtl.DTLVardef@1b0aa4org.highwire.dtl.DTLVardef@157a67aorg.highwire.dtl.DTLVardef@172a2a2org.highwire.dtl.DTLVardef@1136db6_HPS_FORMAT_FIGEXP M_FIG C_FIG