RAPD amplicon sequence based metagenomics analysis of microbial communities associated with clove (Syzygium aromaticum) from Kerala, India
Four clove clones (BRC1, BRC3 MRC5 and MRC7) were collected from different places of ponmudi hills, Kerala, India. DNA was extracted and RAPD analysis was designed to evaluate clove clonal variation. Upon analysis bacterial sequences were also identified among clove genome sequences. So the clove and microbial sequences were separated and performed an analysis to identify clove associated metagenome. The major microbes present were Mycobacterium canettii Xanthomaonas euvesicateria, Klebsiella and Psuedomonas. A total of 88 genus of microbes were present altogether in four clones of Syzygium aromaticum. There were few fungal species were also identified, Fusarium pseudograminearum was the major fungus associated with all four different clones of clove.