Biology subjects

Samadian, H.

Publications and source records attributed to Samadian, H..

3 recordsLinked to original sources

bioRxiv2022

Carbon nanofibers fabrication, surface modifications, and application as the innovative substrate for electrical stimulation of neural cell differentiation

Engineered nanostructures are innovative and precisely designed, synthesized, and tailored with outstanding physicochemical properties that can be applied as the game-changer in neural tissue engineering. The present study aimed to develop an innovative approach based on electrical stimulation through a conductive scaffold to differentiate neural cells from human adipose mesenchymal stem cells without the use of a specific environment for neural differentiation. Electrospun carbon nanofibers (CNFs) were obtained using heat treatment of polyacrylonitrile nanofibers and treated by nitric acid, ethylenediamine, and oxygen Plasma. SEM imaging revealed that the treated nanofibers have s diameter in the range of 120-200 nm and the treatment did not significantly change the CNFs diameter. The FTIR results showed that the treatments were able to introduce COOH, OH, and NH2 functional groups on the CNFS surface. The XRD and Raman analysis showed that the plasma treatment induced the lowest structural changes in the CNFs microstructure. The biocompatibility assessments showed that the pristine and treated CNFs were non-toxic induced proliferative effect on human adipose-derived mesenchymal stem cells. The electrical stimulation (1.5 mA current with a frequency of 500 Hz and CMOS waveform for 7 days 10 min each day) induced the expression of neural genes and proteins by the cells cultured on the treated CNFs. The Plasma-treated CNFs mediated the highest differentiation outcome. These results indicate that electrospun CNFs can be applied as the innovative interface applicable for neural tissue regeneration under electrical stimulation. Research highlightsO_LICNFs were fabricated from PAN nanofibers C_LIO_LIDifferent amounts of ZnONPs were incorporated into or sprayed on CNF C_LIO_LIincreasing in ZnONP amount decreased conductivity, surface wettability was improved by [~]19-33%. C_LIO_LIAlso, FTIR, XRD, and Raman analyses proved that the presence of ZnONP improved structure formation with lower defect density C_LI O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=96 SRC="FIGDIR/small/512333v1_figS1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@a6eb07org.highwire.dtl.DTLVardef@9040e8org.highwire.dtl.DTLVardef@955840org.highwire.dtl.DTLVardef@d4fa0e_HPS_FORMAT_FIGEXP M_FIG O_FLOATNOSchematic 1.C_FLOATNO The applied electrical stimulation setup C_FIG

bioengineering↗

bioRxiv2020

Electro-conductive carbon nanofibers containing ferrous sulfate for bone tissue engineering

The application of electroactive scaffolds can be promising for bone tissue engineering applications. In the current paper, we aimed to fabricate an electro-conductive scaffold based on carbon nanofibers (CNFs) containing ferrous sulfate. FeSO4{middle dot}7H2O salt with different concentrations 5, 10, and 15 wt%, were blended with polyacrylonitrile (PAN) polymer as the precursor and converted to Fe2O3/CNFs nanocomposite by electrospinning and heat treatment. The characterization was conducted using SEM, EDX, XRD, FTIR, and Raman methods. The results showed that the incorporation of Fe salt did not induce an adverse effect on the nanofibers morphology. EDX analysis confirmed that the Fe are uniformly dispersed throughout the CNF mat. FTIR spectroscopy showed the interaction of Fe salt with PAN polymer. Raman spectroscopy showed that the incorporation of FeSO4{middle dot}7H2O reduced the ID/IG ratio, indicating more ordered carbon in the synthesized nanocomposite. Electrical resistance measurement depicted that, although the incorporation of ferrous sulfate reduced the electrical conductivity, the conductive is suitable for electrical stimulation. The in vitro studies revealed that the prepared nanocomposites were cytocompatible and only negligible toxicity (less than 10%) induced by CNFs/Fe2O3 fabricated from PAN FeSO4{middle dot}7H2O 15%. These results showed that the fabricated nanocomposites could be applied as the bone tissue engineering scaffold.

bioinformatics↗

bioRxiv2019

Electrospun cellulose acetate/gelatin nanofibrous wound dressing containing berberine for diabetic foot ulcer healing: in vitro and in vivo studies

Functional dressing with tailored physicochemical and biological properties is vital for diabetic foot ulcer (DFU) treatment. Our main objective in the current study was to fabricate Cellulose Acetate/Gelatin (CA/Gel) electrospun nanofibrous mat loaded with berberine (Beri) as the DFU dressing. The results demonstrated that the diameter of the nanofibers was around 502 nm, the tensile strength, contact angle, porosity, water vapor permeability, and water uptake ratio of CA/Gel nanofibers were around 2.83 MPa, 58.07, 78.17 %, 11.23 mg/cm2 hr, and 12.78 respectively, while these values for CA/Gel/Beri nanofibers were 2.69 {+/-} 0.05 MPa, 56.93 {+/-} 1, 76.17 {+/-} 0.76 %, 10.17 {+/-} 0.21 mg/cm2 hr, 14.37 {+/-} 0.42 respectively. The bacterial evaluations demonstrated that the dressings are an excellent barrier against bacterial penetration with potent antibacterial activity. The animal studies depicted that the collagen density and angiogenesis score in the CA/Gel/Beri treated group were 88.8{+/-}6.7 % and 19.8{+/-}3.8, respectively. These findings implied that the incorporation of berberine did not compromise the physical properties of dressing, while improving the biological activates. In conclusion, our findings implied that the prepared mat is a proper wound dressing for DFU management and treatment.

bioengineering↗