Biology subjects

Salomon, C.

Publications and source records attributed to Salomon, C..

3 recordsLinked to original sources

bioRxiv2025

From Lab to Industry: Awareness of the translational potential of Extracellular Vesicles by the ISEV Translation, Regulation and Advocacy Committee (ISEV-TRA).

Extracellular vesicles (EVs) are critical mediators of cellular communication, with significant potential for disease diagnosis, therapeutics, and other industrial applications. However, translating EV-based innovations into real-world products faces substantial challenges, particularly concerning regulatory frameworks and scientific gaps. To address these issues, the International Society for Extracellular Vesicles (ISEV) established the Translation, Regulation, and Advocacy Committee (ISEV-TRA) to create an environment that facilitates the translation of EV research into practical societal applications. This reporet presents the results of the first survey conducted by the ISEV-TRA, assessing the degree to which EVs have been translated into society applications, awareness of EV potential, and the current use of EVs across various industry sectors. Respondents included individuals from Academia, Industry, and dual affiliation (Academia-Industry), provided an initial perspective on EV research and its translational status. The survey highlights the growing interest in EV-based products, suggesting that there is an important wave of efforts to develop these products. Several spin-outs created by academic researchers are focusing on EV-based products, a key trend in the field, while large companies are showing increasing interest in EV-based technologies. The expectations and suggestions for the newly established ISEV-TRA underscore the need for a unified approach to accelerate the translation of EV-based technologies into practical applications. The ISEV-TRA is well-positioned to play a key role by providing essential resources, organizing workshops, and promoting interdisciplinary collaboration, ultimately driving the commercialization of EV-based innovations.

scientific communication and education↗

bioRxiv2024

In vivo sequential mutagenesis in germinal center B cells using a dual-recombinase approach: FOXO1 re-expression upon FOXO1 knockout rescues class switch recombination

Modeling complex (patho)physiological processes by sequential mutagenesis in mice is limited by the lack of optimized genetic tools and complex breeding strategies. We present a new Cre/DreERT2 dual-recombinase germinal center B-cell (GCBC)- specific strain, with co-expression of the recombinases from a single allele. This enables highly efficient Cre-mediated FOXO1 knockout followed by time-controlled, efficient Dre-mediated FOXO1 re-expression and functional rescue in GCBCs, demonstrating suitability for precise targeted sequential mutagenesis in vivo.

immunology↗

bioRxiv2023

Whole transcriptome profiling of placental pathobiology in SARS-CoV-2 pregnancies identifies a preeclampsia-like gene signature

ObjectivesSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus infection in pregnancy is associated with higher incidence of placental dysfunction, referred to by a few studies as a "preeclampsia-like syndrome". However, the mechanisms underpinning SARS-CoV-2-induced placental malfunction are still unclear. Here, we investigated whether the transcriptional architecture of the placenta is altered in response to SARS-CoV-2 infection. MethodsWe utilized whole-transcriptome, digital spatial profiling, to examine gene expression patterns in placental tissues from participants who contracted SARS-CoV-2 in the third trimester of their pregnancy (n=7) and those collected prior to the start of the coronavirus disease 2019 (COVID-19) pandemic (n=9). ResultsThrough comprehensive spatial transcriptomic analyses of the trophoblast and villous core stromal cell subpopulations in the placenta, we identified signatures associated with hypoxia and placental dysfunction during SARS-CoV-2 infection in pregnancy. Notably, genes associated with vasodilation (NOS3), oxidative stress (GDF15, CRH), and preeclampsia (FLT1, EGFR, KISS1, PAPPA2), were enriched with SARS-CoV-2. Pathways related to increased nutrient uptake, vascular tension, hypertension, and inflammation, were also enriched in SARS-CoV-2 samples compared to uninfected controls. ConclusionsOur findings demonstrate the utility of spatially resolved transcriptomic analysis in defining the underlying pathogenic mechanisms of SARS-CoV-2 in pregnancy, particularly its role in placental dysfunction. Furthermore, this study highlights the significance of digital spatial profiling in mapping the intricate crosstalk between trophoblasts and villous core stromal cells, thus shedding light on pathways associated with placental dysfunction in pregnancies with SARS-CoV-2 infection. Graphical abstractIn this study, using spatial digital profiling transcriptomic approaches, we demonstrate that SARS-CoV-2 infection in pregnancy disrupts optimal placental function by altering the genomic architecture of trophoblasts and villous core stromal cells.

developmental biology↗