Biology subjects

Salimnia, H.

Publications and source records attributed to Salimnia, H..

3 recordsLinked to original sources

bioRxiv2024

Identification and mitigation of blood's interference with the antimicrobial activity of AgNbO3 particles

The detrimental impact of blood on the antimicrobial activity of AgNbO3 particles was identified and investigated. It was observed that the impact is more severe in the case of lysed blood and also operates in the case of commonly used silver salt, AgNO3. The inhibition was shown to be due to hemoglobin, but unrelated to the heme moiety. In an attempt to find additives to mitigate the inhibitory effect of hemoglobin, iron ions and the chelating agent, K2EDTA, were selected as promising candidates. Including iron on the particles was shown to have a marginal effect, but supplying the medium with K2EDTA, an agent preventing clotting of blood samples, fully countered the deleterious impact of hemoglobin on AgNbO3 activity. These findings may be relevant for adapting the silver compounds to applications such as wound dressings, where silvers antimicrobial action would have to take place in a medium containing blood.

microbiology↗

bioRxiv2024

Bacterial growth dynamics on a surface having a particulate antimicrobial agent

The morphological dynamics of microbial cell proliferation on an antimicrobial surface at an early growth stage was studied with Escherichia coli on the surface of a gel supplied with nanostructured AgNbO3 antimicrobial particles. We demonstrated an inhibitory surface concentration, analogous to minimum inhibitory concentration, beyond which the growth of colonies and formation of biofilm are inhibited. In contrast, at lower concentrations, colonies circumvent the antimicrobial activity of the particles and grow with a short lag time of a few hours. The applicability of these findings, in terms of estimating inhibitory surface concentration, was tested in the case of antimicrobial polymethyl methacrylate (PMMA) bone cement.

bioengineering↗

bioRxiv2017

Automated collection of pathogen-specific diagnostic data for real-time syndromic epidemiological studies

Health-care and public health professionals rely on accurate, real-time monitoring of infectious diseases for outbreak preparedness and response. Early detection of outbreaks is improved by systems that are pathogen-specific. We describe a system, FilmArray(R) Trend, for rapid disease reporting that is syndrome-based but pathogen-specific. Results from a multiplex molecular diagnostic test are sent directly to a cloud database. www.syndromictrends.com presents these data in near real-time. Trend preserves patient privacy by removing or obfuscating patient identifiers. We summarize the respiratory pathogen results, for 20 organisms from 344,000 patient samples acquired as standard of care testing over the last four years from 20 clinical laboratories in the United States. The majority of pathogens show influenza-like seasonality, rhinovirus has fall and spring peaks and adenovirus and bacterial pathogens show constant detection over the year. Interestingly, the rate of pathogen co-detections, on average 7.7%, matches predictions based on the relative abundance of organisms present.

epidemiology↗