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SINGH, M.

Publications and source records attributed to SINGH, M..

3 recordsLinked to original sources

Environmental impacts of Broiler chicken production in the North Eastern Himalayan region of India: Evaluation using the Life Cycle Assessment approach

The study presents the first comprehensive environmental assessment of broiler chicken production in India, from a cradle-to-farm-gate perspective, using the Life Cycle Assessment (LCA) approach. The objective of the study was to identify environmental hotspots in the broiler production system. All inflows and outflows of the broiler production system were mapped to construct the life-cycle inventory. This study uses a system boundary that extends from the cradle to the farm gate. Inventory data for the broiler chicken farms were collected from six different farms, which is typical of the Indian broiler production system, and background data were sourced from the Ecoinvent 3.0 database. For the LCA study, SimaPro (v 9.3.0.3) was used with the ReCiPe 2016 Midpoint impact assessment methodology. For this study, the functional unit of the "1-kg live weight chicken produced was taken into consideration". The environmental impact categories assessed were mainly Global Warming Potential in hundred years (GWP), stratospheric ozone depletion, freshwater eutrophication, terrestrial ecotoxicity, land use, etc. Results showed that broiler chicken feed was primarily responsible for environmental impacts, followed by transportation and electricity. Broiler chicken production had a total GWP of 3.77 kg CO2-eq per kilogram of live weight. Specifically, the energy component of feed, viz., maize production, was the main source of environmental impact. The process of transporting feed and chicks to the broiler farm also had a significant environmental impact. The broiler production system was found to have moderate environmental impacts compared to other published LCA studies of chicken production. Based on the findings of the present study, we proposed actionable items to further improve the environmental efficiency of broiler chicken production in India.

ecology↗

PROTEOME DATA BASED IDENTIFICATION OF POTENTIAL RNAi TARGETS FOR COTTON MEALYBUG (Phenacoccus solenopsis Tinsley) POPULATION MANAGEMENT

Background of the studyPhenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae), commonly known as cotton mealybug, regarded as an invasive pest worldwide, particularly in the tropics and subtropics. It is one of the major pests of cotton and other commercially important crops. Despite the significant economic losses caused by cotton mealybug the molecular aspects of this insect are under-studied. MethodsIn the present study, proteome data of four different developmental stages of cotton mealybug is generated. Differential expression of proteins (DEPs) was studied among six different groups of which, maximum DEPs (550 up-regulated and 1118 down-regulated) were obtained when the quantifiable proteins of Egg+first nymphal were compared with second nymphal instar (FC [&ge;] 2, P < 0.05). From this proteomics data fifteen potential target genes were predicted for insect pest management. Further, these fifteen genes were explored and evaluated the for RNAi based pest control and optimisation of dsRNA delivery system in cotton mealybug. The analysis of transient expression of target genes was performed. ResultsThe results signified that dsRNA of Ferritin caused [~]69% mortality hence, could be exploited as a promising candidate gene to design a sustainable method for cotton mealybug management. ConclusionThis study provides an urgently required, alternate green control strategy based on proteomics to identify potential RNAi targets for pest management.

molecular biology↗

Single cell profiling of functionally cured Chronic Hepatitis B patients reveals the emergence of activated innate and an altered adaptive immune response in the intra- hepatic envrionment

Hepatitis B surface antigen (HBsAg) loss or functional cure (FC), is considered the desirable therapeutic outcome for chronic hepatitis B (CHB) patients. However, the immuno-pathological biomarkers and underlying mechanisms remain unclear. Here we present a comprehensive single cell-transcriptomic atlas together with immune-phenotyping of disease-associated cell states (DACS) isolated from intra-hepatic tissue and matched PBMCs of either CHB or FC patients. We find that the intra-hepatic environment displays specific cell identities and molecular signatures that are distinct from PBMCs. FC is associated with emergence of an altered adaptive immune response marked by CD4 cytotoxic T lymphocytes (CD4-CTLs), and an activated innate response represented by liver-resident natural killer (LR-NK) cells. Overall, these findings provide novel insights into immuno-pathological cell states associated with FC that could serve as prognostic biomarkers.

genomics↗