S, S. R. | bioRxiv Science

bioRxiv2023

Spatio-temporal evaluation of drought adaptation in wheat revealed NDVI and MTSI as powerful tools for selecting tolerant genotypes

ContextWater stress is fast becoming a major limiting factor for wheat production. Hence, identifying drought tolerant genotypes is critical for sustaining the food supply chain. However, there are no phenotypic markers or statistical models available that may be employed for the efficient selection of field grown drought tolerant wheat genotypes. ObjectivesWe evaluated wheat genotypes to (1) identify novel sources of drought tolerance (2) understand underlying adaptation mechanisms of drought tolerance (3) identify phenotypic markers and a stable model for the selection of drought tolerant genotypes MethodsOne hundred ninety-six diverse wheat genotypes were evaluated at three different locations in India: Banaras Hindu University (BHU), Varanasi (E1 and E3: control; E2 and E4: drought); Agharkar Research Institute (ARI), Pune (E5 and E7: control; E6 and E8: drought) and Borlaug Institute for South Asia (BISA), Jabalpur (E9 and E11: control; E10 and E12: drought) for various agronomic, physiological and yield traits for two consecutive years. Drought was imposed at the heading stage (Z59) by withholding irrigation for four weeks until the moisture reading reached <45% than the control (100%). ResultsThe performance of all genotypes significantly declined under drought at all the locations. Normalized difference vegetation index (NDVI) significantly correlated (r = 0.41** and 0.36**) with the grain yield under drought during maturity. At the same time, there was no association under control conditions (r = 0.07 and 0.10) at the BHU center during 2020-21 and 2021-22, respectively. Stress indices, such as geometric mean productivity (GMP) and stress tolerance index (STI), showed a high correlation (r= 0.89** and r = 0.88**, respectively) with the grain yield under drought and were effective in differentiating drought tolerant genotypes. GGE bi-plots discriminated the environments (observed obtuse angle between E3 with E6 and E9, E4 with E6 and E9) having negative relation and cross-over interaction for grain yield. Further, the multi-trait stability index (MTSI) identified 29 stable genotypes across all environments and was predicted as the most accurate model due to its fewer Root Mean Square Prediction Difference (RMSPD) values. ConclusionNDVI is a useful high throughput screening tool under drought and MTSI is an effective method for selecting stable wheat genotypes across different water stressed locations. ImplicationsThe identified tools (NDVI), method (MTSI), and tolerant genotypes appear to be valuable resources that together will be useful in the ongoing breeding programs to enhance the drought tolerance of wheat. HighlightsO_LIDrought stress significantly reduces wheat production globally. C_LIO_LINDVI serves as a useful tool for high throughput screening under drought for field-based experiments. C_LIO_LIAMMI, GGE, WAASBY and MTSI plots are effective in detecting GEI effects. C_LIO_LIMTSI is a reliable tool for selecting stable and high mean performers. C_LI

plant biology↗

bioRxiv2022

PHYSIOLOGICAL CHARACTERISTICS OF BACTERIAL DROPLETS INDICATE A CATASTROPHIC CONSEQUENCE WITH AN INCREASE IN IMPACT VELOCITY

Droplet impacts on various surfaces play a profound role in different bio-physiological processes and engineering applications. The current study opens a new realm that investigates the plausible effect of impact velocities on bacteria-laden droplets against a solid surface. We unveiled the alarming consequences of Salmonella Typhimurium (STM) laden drop, carrying out the in vitro and intracellular viability of STM to the impact Weber numbers ranging from 100-750. The specified Weber number range mimics the velocity range occurring during the respiratory processes, especially the airborne dispersion of drops during cough. A thick ring of bacterial deposition was observed in all cases irrespective of impacting velocity and the nutrient content of the bacterial medium. The mechanical properties of the bacterial deposit examined using Atomic Force Microscopy reveals the deformation of bacterial morphology, cushioning effect and adhesion energy to determine the cell-cell interactions. The impact velocity induces the shear stress onto the cell walls of STM, thereby deteriorating the in vitro viability. However, we found that even with compromised in vitro viability, Salmonella retrieved from deposited patterns impacted at higher velocity revealed an increased expression of phoP (the response regulator of the PhopQ two-component system) and uninterrupted intracellular proliferation in macrophages. The inability of STM{Delta} phoP growth in nutrient-rich dried droplets to the subjected impact velocities signifies the predominant role of phoP in maintaining the virulence of Salmonella during desiccation stress. Our findings open a promising avenue for understating the effect of bacteria-laden drop impact and its role in disease spread. O_FIG_DISPLAY_L [Figure 1] M_FIG_DISPLAY C_FIG_DISPLAY

bioengineering↗